LOCUS       sequence-164162-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Induced by acetoacetate to express GFP using atoSC two-component 
            system in E. coli. Contains the atoDAEB promoter (Pato) with a 
            strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Rutter JW, Dekker L, Fedorec AJH, Gonzales DT, Wen KY, Tanner LES, 
            Donovan E, Ozdemir T, Thomas GM, Barnes CP
  TITLE     Engineered acetoacetate-inducible whole-cell biosensors based on the
            AtoSC two-component system.
  JOURNAL   Biotechnol Bioeng. 2021 Nov;118(11):4278-4289. doi: 
            10.1002/bit.27897. Epub 2021 Aug 9.
  PUBMED    34289076
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.27897";
            journalName: "Biotechnol Bioeng"; date: "2021-11"; volume: "118"; 
            issue: "11"; pages: "4278-4289"
COMMENT     Sequence Label: PatoM-OG241
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    282..353
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(398..534)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(564..610)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgattt
      121 aaatgaaact cgtaatcgga gaacgggtgc aatagcaagg accagatcga acttgactga
      181 cgcaattctg agcgtagtcg aaacccagac aagtcgatct cgccaagtag ctgacttgac
      241 tggttaatta agtttcgata gcccaaggta accaatgaga ttagggaggg aagaaagttt
      301 gatgaactac ttctgacccc tagtggtgtc cagaaaagac cattaaagga atgtcttgat
      361 cgaaatcgag agatcgcctg caggatagct gacgacctta aggataaatt tctggtaagg
      421 aggacacgta tggaagtggg caagttgggg aagccgtatc cgttgctgaa tctggcatat
      481 gtgggagtat aagacgcgca gcgtcgcatc aggcattttt ttctgcgcca atgccggtag
      541 atagcaatcc ggcccgaggg gcacaaaaaa cccctcaaga cccgtttaga ggccccaagg
      601 ggttatgcta cgataccgat agagatgggc caataccaca tttgtagagg ttttacttgc
      661 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
      721 tgttaacttg tttat
//
