LOCUS       sequence-164165-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Induced by acetoacetate to express GFP using atoSC two-component 
            system in E. coli. Contains the atoDAEB promoter (Pato) with a 
            strong RBS, Bba_B0034 (iGEM). LOG241 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Rutter JW, Dekker L, Fedorec AJH, Gonzales DT, Wen KY, Tanner LES, 
            Donovan E, Ozdemir T, Thomas GM, Barnes CP
  TITLE     Engineered acetoacetate-inducible whole-cell biosensors based on the
            AtoSC two-component system.
  JOURNAL   Biotechnol Bioeng. 2021 Nov;118(11):4278-4289. doi: 
            10.1002/bit.27897. Epub 2021 Aug 9.
  PUBMED    34289076
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.27897";
            journalName: "Biotechnol Bioeng"; date: "2021-11"; volume: "118"; 
            issue: "11"; pages: "4278-4289"
COMMENT     Sequence Label: PatoL-LOG241
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             263..274
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ttctctgtcg accaactagt gccgtgcatt gatgtataaa ctccaggaat acggtatcga
       61 tccggcggat gtataacacc aaaacttgct atgcagaaat ttgcacagtg cgcaattttc
      121 tgcatagccg ctcattctcc ttataaatcc ccatccaatt tatcccttca tattcaatta
      181 gttaaataac taaatccaat aatctcattc tggcactccc cttgctattg cctgactgta
      241 cccacaacgg tgtatgcaga ttaaagagga gaaatactag atggtaccca ccatgacagc
      301 tctgacagag ggagcgaaac tgttcgagaa ggagatcccg tacatcacag agttggaagg
      361 agatgtcgaa gggatgaaat tcatcatcaa gggagaagga acgggtgacg caacgaccgg
      421 aacgatcaag gccaaataca tttgtaccac gggcgatttg cctgtcccct gggcgacgct
      481 tgtaagcacg ctctcgtatg gtgtccagtg cttcgcgaaa tatccatcgc acattaagga
      541 ctttttcaag tcggccatgc cagaaggtta cacacaagaa cgaaccatct cctttgaggg
      601 ggacggagtg tataagacac gcgcgatggt aacgtacgag cgcgggtcca tctacaacag
      661 ggtaactctt actggggaga actttaagaa ggacgggcat atcttgcgga aaaacgtggc
      721 atttcaatgt ccgccctcga ttctgtatat tctcccggac acggtgaata atgggatcag
      781 agtggagttc aaccaggcat
//
