LOCUS       sequence-164166-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Induced by acetoacetate to express GFP using atoSC two-component 
            system in E. coli. Contains the atoDAEB promoter (Pato) with a 
            strong RBS, Bba_B0034 (iGEM). LOG241 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Rutter JW, Dekker L, Fedorec AJH, Gonzales DT, Wen KY, Tanner LES, 
            Donovan E, Ozdemir T, Thomas GM, Barnes CP
  TITLE     Engineered acetoacetate-inducible whole-cell biosensors based on the
            AtoSC two-component system.
  JOURNAL   Biotechnol Bioeng. 2021 Nov;118(11):4278-4289. doi: 
            10.1002/bit.27897. Epub 2021 Aug 9.
  PUBMED    34289076
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.27897";
            journalName: "Biotechnol Bioeng"; date: "2021-11"; volume: "118"; 
            issue: "11"; pages: "4278-4289"
COMMENT     Sequence Label: PatoL-LOG241
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    238..309
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(354..490)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(520..566)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa caggcttacc
       61 cgtcttactg tcatttaaat gaaactcgta atcggagaac gggtgcaata gcaaggacca
      121 gatcgaactt gactgacgca attctgagcg tagtcgaaac ccagacaagt cgatctcgcc
      181 aagtagctga cttgactggt taattaagtt tcgatagccc aaggtaacca atgagattag
      241 ggagggaaga aagtttgatg aactacttct gacccctagt ggtgtccaga aaagaccatt
      301 aaaggaatgt cttgatcgaa atcgagagat cgcctgcagg atagctgacg accttaagga
      361 taaatttctg gtaaggagga cacgtatgga agtgggcaag ttggggaagc cgtatccgtt
      421 gctgaatctg gcatatgtgg gagtataaga cgcgcagcgt cgcatcaggc atttttttct
      481 gcgccaatgc cggtagatag caatccggcc cgaggggcac aaaaaacccc tcaagacccg
      541 tttagaggcc ccaaggggtt atgctacgat accgatagag atgggccaat accacatttg
      601 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
      661 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
      721 atagcatcac aaatt
//
