LOCUS       sequence-164167-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Induced by acetoacetate to express GFP using atoSC two-component 
            system in E. coli. Contains the atoDAEB promoter (Pato) with a 
            strong RBS, Shine-Dalgarno sequence provided in OG241. LOG241 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Rutter JW, Dekker L, Fedorec AJH, Gonzales DT, Wen KY, Tanner LES, 
            Donovan E, Ozdemir T, Thomas GM, Barnes CP
  TITLE     Engineered acetoacetate-inducible whole-cell biosensors based on the
            AtoSC two-component system.
  JOURNAL   Biotechnol Bioeng. 2021 Nov;118(11):4278-4289. doi: 
            10.1002/bit.27897. Epub 2021 Aug 9.
  PUBMED    34289076
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.27897";
            journalName: "Biotechnol Bioeng"; date: "2021-11"; volume: "118"; 
            issue: "11"; pages: "4278-4289"
COMMENT     Sequence Label: PatoM-LOG241
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttctctgtc gaccaactag tgccgtgcat tgatgtataa actccaggaa tacggtatcg
       61 atccggcgga tgtataacac caaaacttgc tatgcagaaa tttgcacagt gcgcaatttt
      121 ctgcatagcc gctcattctc cttataaatc cccatccaat ttatcccttc atattcaatt
      181 agttaaataa ctaaatccaa taatctcatt ctggcactcc ccttgctatt gcctgactgt
      241 acccacaacg gtgtatgcag aattcaaagg aggtacccac catgacagct ctgacagagg
      301 gagcgaaact gttcgagaag gagatcccgt acatcacaga gttggaagga gatgtcgaag
      361 ggatgaaatt catcatcaag ggagaaggaa cgggtgacgc aacgaccgga acgatcaagg
      421 ccaaatacat ttgtaccacg ggcgatttgc ctgtcccctg ggcgacgctt gtaagcacgc
      481 tctcgtatgg tgtccagtgc ttcgcgaaat atccatcgca cattaaggac tttttcaagt
      541 cggccatgcc agaaggttac acacaagaac gaaccatctc ctttgagggg gacggagtgt
      601 ataagacacg cgcgatggta acgtacgagc gcgggtccat ctacaacagg gtaactctta
      661 ctggggagaa ctttaagaag gacgggcata tcttgcggaa aaacgtggca tttcaatgtc
      721 cgccctcgat tctgtatatt ctcccggaca cggtga
//
