LOCUS       sequence-164168-         520 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Induced by acetoacetate to express GFP using atoSC two-component 
            system in E. coli. Contains the atoDAEB promoter (Pato) with a 
            strong RBS, Shine-Dalgarno sequence provided in OG241. LOG241 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Rutter JW, Dekker L, Fedorec AJH, Gonzales DT, Wen KY, Tanner LES, 
            Donovan E, Ozdemir T, Thomas GM, Barnes CP
  TITLE     Engineered acetoacetate-inducible whole-cell biosensors based on the
            AtoSC two-component system.
  JOURNAL   Biotechnol Bioeng. 2021 Nov;118(11):4278-4289. doi: 
            10.1002/bit.27897. Epub 2021 Aug 9.
  PUBMED    34289076
REFERENCE   2  (bases 1 to 520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.27897";
            journalName: "Biotechnol Bioeng"; date: "2021-11"; volume: "118"; 
            issue: "11"; pages: "4278-4289"
COMMENT     Sequence Label: PatoM-LOG241
FEATURES             Location/Qualifiers
     source          1..520
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    233..304
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(349..485)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 cattcagact ggctaatgca cccagtaagg cagcggtatc atcaacaggc ttacccgtct
       61 tactgtcatt taaatgaaac tcgtaatcgg agaacgggtg caatagcaag gaccagatcg
      121 aacttgactg acgcaattct gagcgtagtc gaaacccaga caagtcgatc tcgccaagta
      181 gctgacttga ctggttaatt aagtttcgat agcccaaggt aaccaatgag attagggagg
      241 gaagaaagtt tgatgaacta cttctgaccc ctagtggtgt ccagaaaaga ccattaaagg
      301 aatgtcttga tcgaaatcga gagatcgcct gcaggatagc tgacgacctt aaggataaat
      361 ttctggtaag gaggacacgt atggaagtgg gcaagttggg gaagccgtat ccgttgctga
      421 atctggcata tgtgggagta taagacgcgc agcgtcgcat caggcatttt tttctgcgcc
      481 aatgccggta gatagcaatc cggcccgagg ggcacaaaaa
//
