LOCUS       sequence-164172-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NOT induced by acetoacetate to express GFP. Contains the native 
            atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP
            gene. LOG241 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Rutter JW, Dekker L, Fedorec AJH, Gonzales DT, Wen KY, Tanner LES, 
            Donovan E, Ozdemir T, Thomas GM, Barnes CP
  TITLE     Engineered acetoacetate-inducible whole-cell biosensors based on the
            AtoSC two-component system.
  JOURNAL   Biotechnol Bioeng. 2021 Nov;118(11):4278-4289. doi: 
            10.1002/bit.27897. Epub 2021 Aug 9.
  PUBMED    34289076
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.27897";
            journalName: "Biotechnol Bioeng"; date: "2021-11"; volume: "118"; 
            issue: "11"; pages: "4278-4289"
COMMENT     Sequence Label: PatoK-LOG241
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    243..314
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(359..495)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(525..571)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(723..742)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cgtgacaggt cattcagact ggctaatgca cccagtaagg cagcggtatc atcaacaggc
       61 ttacccgtct tactgtcatt taaatgaaac tcgtaatcgg agaacgggtg caatagcaag
      121 gaccagatcg aacttgactg acgcaattct gagcgtagtc gaaacccaga caagtcgatc
      181 tcgccaagta gctgacttga ctggttaatt aagtttcgat agcccaaggt aaccaatgag
      241 attagggagg gaagaaagtt tgatgaacta cttctgaccc ctagtggtgt ccagaaaaga
      301 ccattaaagg aatgtcttga tcgaaatcga gagatcgcct gcaggatagc tgacgacctt
      361 aaggataaat ttctggtaag gaggacacgt atggaagtgg gcaagttggg gaagccgtat
      421 ccgttgctga atctggcata tgtgggagta taagacgcgc agcgtcgcat caggcatttt
      481 tttctgcgcc aatgccggta gatagcaatc cggcccgagg ggcacaaaaa acccctcaag
      541 acccgtttag aggccccaag gggttatgct acgataccga tagagatggg ccaataccac
      601 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
      661 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
      721 aagcaatagc atcacaaatt tcacaaataa agcatttt
//
