LOCUS       sequence-164174-        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing SLC35B2-p2A-E2Crimson.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, 
            Monel B, Schumann K, Yu H, Krupzcak KM, Garcia-Beltran W, 
            Piechocka-Trocha A, Krogan NJ, Marson A, Sabatini DM, Lander ES, 
            Hacohen N, Walker BD
  TITLE     A genome-wide CRISPR screen identifies a restricted set of HIV host 
            dependency factors.
  JOURNAL   Nat Genet. 2016 Dec 19. doi: 10.1038/ng.3741.
  PUBMED    27992415
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2016 Dec 19. doi: 10.1038/ng.3741."
COMMENT     Sequence Label: SLC35B2-p2A-E2Crimson
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..90)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 catgaagggc ttgatgacgt tctcagtgct attggggccg gggttctcct ccacgtcgcc
       61 ggcctgcttc agcaggctga aatttgtagc tcctccacct cctgcggccg ccaccttctg
      121 cacaggactt tccaccggga cggctttttt tcctctttgc ttaagccttc ccctagcgta
      181 tacgcgcagc aggagtgctg cgaacacaac ggccacaccc agacctccta cgacagttac
      241 ggtatggccg tagaggagac agctgaggag gatagcgaat gcctgcctca gagtcataat
      301 tattgtgaac acagcggccc caaattgacc gatggtgtaa aagatgaaga gctgcccgca
      361 agcactgcaa atactcagca gcagagcgtg agcggcaaac tcggaatgtc tgcccataaa
      421 tcttgttccc tcaagcagcg ccccttgttc cagaagactg cccacggtga acaagcagct
      481 aaagaaattc accccaaaca tcatctgcac agaggacatc ttgtaggcga acaaggcatc
      541 ctgccaatta cttgtaaaag aatcgaaggc gatatagccg gccagcagga tcagtccgct
      601 aagggttgtt gcagggctgc tccttggttc gggcccactt gacagcaaaa acattgagac
      661 tccaatactg ataagggtcg ccgtcaggta ttcccaatgc tcgtatgagc gccgggatac
      721 cagtttcccc atcagcatca ccggaatgac tttactggct ttggcgagca cttgggtagg
      781 aaagctgaca aatttaagag cctcgtactg acaccagcta gacagtacgt tgctcagtga
      841 tgcgaaacta tagcggtaca tcggggctcc atgcctgggt tgtttacaca gcacacatga
      901 cagaccggcg acgatcagcg ccagcacccg attcatgagc acgaggaatt gagagtctgt
      961 gaaccgctct ccgggggagg tggcggtggc gccgtaggac cgagtcatga cgcgttcctg
     1021 aaggaccccc catgtcaggt a
//
