LOCUS       sequence-164177-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein 
            folding chaperones..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Lamppa JW, Tanyos SA, Griswold KE
  TITLE     Engineering Escherichia coli for soluble expression and single step 
            purification of active human lysozyme.
  JOURNAL   J Biotechnol. 2013 Mar 10;164(1):1-8. doi: 
            10.1016/j.jbiotec.2012.11.007. Epub 2012 Dec 7.
  PUBMED    23220215
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jbiotec.2012.11.007"; journalName: "J Biotechnol"; date: 
            "2013-03-10- 10"; volume: "164"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pACYC-Skp-GroEL/ES
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        104..122
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    123..147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        689..707
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    708..732
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg
       61 ggatctcgac gctctccctt atgcgactcc tgcattagga aattaatacg actcactata
      121 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
      181 gagatatacc atggctgaca aaattgcaat cgtcaacatg ggcagcctgt tccagcaggt
      241 agcgcagaaa accggtgttt ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga
      301 actgcagcgt atggaaaccg atctgcaggc taaaatgaaa aagctgcagt ccatgaaagc
      361 gggcagcgat cgcactaagc tggaaaaaga cgtgatggct cagcgccaga cttttgctca
      421 gaaagcgcag gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact
      481 ggttactcgt atccagactg ctgtgaaatc cgttgccaac agccaggata tcgatctggt
      541 tgttgatgca aacgccgttg cttacaacag cagcgatgta aaagacatca ctgccgacgt
      601 actgaaacag gttaaataag cttgcggccg cataatgctt aagtcgaaca gaaagtaatc
      661 gtattgtaca cggccgcata atcgaaatta atacgactca ctatagggga attgtgagcg
      721 gataacaatt ccccatctta gtatattagt taagtataag aaggagatat acatatgaat
      781 attcgtccat tgcatgatcg cgtgatcgtc aagcgtaaag aagttgaa
//
