LOCUS       sequence-164178-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor 
            protein folding chaperones..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Lamppa JW, Tanyos SA, Griswold KE
  TITLE     Engineering Escherichia coli for soluble expression and single step 
            purification of active human lysozyme.
  JOURNAL   J Biotechnol. 2013 Mar 10;164(1):1-8. doi: 
            10.1016/j.jbiotec.2012.11.007. Epub 2012 Dec 7.
  PUBMED    23220215
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jbiotec.2012.11.007"; journalName: "J Biotechnol"; date: 
            "2013-03-10- 10"; volume: "164"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pACYC-GroEL/ES-TF
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     105..124
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        105..123
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    124..148
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             240..293
                     /codon_start=1
                     /product="GroES chaperone mobile loop that interacts with 
                     GroEL"
                     /label=S loop
                     /translation="EVETKSAGGIVLTGSAAA"
ORIGIN
        1 ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc
       61 gggatctcga cgctctccct tatgcgactc ctgcattagg aaattaatac gactcactat
      121 aggggaattg tgagcggata acaattcccc tgtagaaata attttgttta actttaataa
      181 ggagatatac catggggaat attcgtccat tgcatgatcg cgtgatcgtc aagcgtaaag
      241 aagttgaaac taaatctgct ggcggcatcg ttctgaccgg ctctgcagcg gctaaatcca
      301 cccgcggcga agtgctggct gtcggcaatg gccgtatcct tgaaaatggc gaagtgaagc
      361 cgctggatgt gaaagttggc gacatcgtta ttttcaacga tggctacggt gtgaaatctg
      421 agaagatcga caatgaagaa gtgttgatca tgtccgaaag cgacattctg gcaattgttg
      481 aagcgtaatc cgcgcacgac actgaacata cgaatttaag gaataaagat aatggcagct
      541 aaagacgtaa aattcggtaa cgacgctcgt gtgaaaatgc tgcgcggcgt aaacgtactg
      601 gcagatgcag tgaaagttac cctcggtcca aaaggccgta acgtagttct ggataaatct
      661 ttcggtgcac cgaccatcac caaagatggt gtttccgttg ctcgtgaaat cgaactggaa
      721 gacaagttcg aaaatatggg tgcgcagatg gtgaaagaag ttgcctctaa agca
//
