LOCUS       sequence-164192-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 with sgRNA targeting Exon 1 of VEGF-A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Yiu G, Tieu E, Nguyen AT, Wong B, Smit-McBride Z
  TITLE     Genomic Disruption of VEGF-A Expression in Human Retinal Pigment 
            Epithelial Cells Using CRISPR-Cas9 Endonuclease.
  JOURNAL   Invest Ophthalmol Vis Sci. 2016 Oct 1;57(13):5490-5497. doi: 
            10.1167/iovs.16-20296.
  PUBMED    27768202
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1167/iovs"; 
            journalName: "Invest Ophthalmol Vis Sci"; date: "2016-10-1- 1"; 
            volume: "57"; issue: "13"; pages: "5490-5497"
COMMENT     Sequence Label: LCV2 V-3
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     114..133
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        213..288
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        350..561
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      580..589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta
       61 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
      121 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
      181 gacgaaacac cgcgagcgcc gagtcgccac tggttttaga gctagaaata gcaagttaaa
      241 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgaattc
      301 gctagctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc
      361 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgatc cggtgcctag
      421 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
      481 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
      541 gggtttgccg ccagaacaca ggaccggttc tagagcgctg ccaccatgga caagaagtac
      601 agcatcggcc tggacatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac
      661 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag
      721 aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag
      781 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc
      841 ttcagcaacg agatggcca
//
