LOCUS       sequence-164193-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 with sgRNA targeting Exon 1 of VEGF-A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Yiu G, Tieu E, Nguyen AT, Wong B, Smit-McBride Z
  TITLE     Genomic Disruption of VEGF-A Expression in Human Retinal Pigment 
            Epithelial Cells Using CRISPR-Cas9 Endonuclease.
  JOURNAL   Invest Ophthalmol Vis Sci. 2016 Oct 1;57(13):5490-5497. doi: 
            10.1167/iovs.16-20296.
  PUBMED    27768202
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1167/iovs"; 
            journalName: "Invest Ophthalmol Vis Sci"; date: "2016-10-1- 1"; 
            volume: "57"; issue: "13"; pages: "5490-5497"
COMMENT     Sequence Label: LCV2 V-3
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(46..294)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(104..123)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(274..294)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    345..462
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(760..804)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acttgctatt tctagctcta aaaccagtgg cgactcggcg ctcgcggtgt ttcgtccttt
       61 ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa gcatatgata
      121 gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt actttctacg
      181 tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattctaa ttatctctct
      241 aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc cctcggtacc
      301 ttaattaacc aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt
      361 gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt
      421 tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat gaatactgcc
      481 atttgtctgc agaattggcg cacgcagtgc cgatccgttc actaatcgaa tggatctgtc
      541 tctgtctctc tctccacctt cttcttctat tccttcgggc ctgtcgggtc ccctcggggt
      601 tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga
      661 aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt
      721 tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa
      781 ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg
      841 tattaagctt gtgtaattgt taatttctct gtcccactcc
//
