LOCUS       sequence-164194-         459 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 with sgRNA targeting Exon 1 of VEGF-A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Yiu G, Tieu E, Nguyen AT, Wong B, Smit-McBride Z
  TITLE     Genomic Disruption of VEGF-A Expression in Human Retinal Pigment 
            Epithelial Cells Using CRISPR-Cas9 Endonuclease.
  JOURNAL   Invest Ophthalmol Vis Sci. 2016 Oct 1;57(13):5490-5497. doi: 
            10.1167/iovs.16-20296.
  PUBMED    27768202
REFERENCE   2  (bases 1 to 459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1167/iovs"; 
            journalName: "Invest Ophthalmol Vis Sci"; date: "2016-10-1- 1"; 
            volume: "57"; issue: "13"; pages: "5490-5497"
COMMENT     Sequence Label: LCV2 V-4
FEATURES             Location/Qualifiers
     source          1..459
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     121..140
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        220..295
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
       61 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
      121 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      181 tggaaaggac gaaacaccgg cccgagctag cacttctcgg ttttagagct agaaatagca
      241 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
      301 tgaattcgct agctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      361 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgatccgg
      421 tgcctagaga aggtggcgcg gggtaaactg ggaaagtga
//
