LOCUS       sequence-164197-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 with sgRNA targeting Exon 1 of VEGF-A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Yiu G, Tieu E, Nguyen AT, Wong B, Smit-McBride Z
  TITLE     Genomic Disruption of VEGF-A Expression in Human Retinal Pigment 
            Epithelial Cells Using CRISPR-Cas9 Endonuclease.
  JOURNAL   Invest Ophthalmol Vis Sci. 2016 Oct 1;57(13):5490-5497. doi: 
            10.1167/iovs.16-20296.
  PUBMED    27768202
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1167/iovs"; 
            journalName: "Invest Ophthalmol Vis Sci"; date: "2016-10-1- 1"; 
            volume: "57"; issue: "13"; pages: "5490-5497"
COMMENT     Sequence Label: LCV2 V-5
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(45..293)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(103..122)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(273..293)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    344..461
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(759..803)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cttgctattt ctagctctaa aactccatga gcccggcttc cgacggtgtt tcgtcctttc
       61 cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggtaag catatgatag
      121 tccattttaa aacataattt taaaactgca aactacccaa gaaattatta ctttctacgt
      181 cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattctaat tatctctcta
      241 acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc ctcggtacct
      301 taattaacca aactggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg
      361 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
      421 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
      481 tttgtctgca gaattggcgc acgcagtgcc gatccgttca ctaatcgaat ggatctgtct
      541 ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt
      601 gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa
      661 agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat gaataatttt
      721 atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat
      781 tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt
      841 attaagcttg tgtaattgtt a
//
