LOCUS       sequence-164199-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable knockout.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Pacold ME, Brimacombe KR, Chan SH, Rohde JM, Lewis CA, Swier LJ, 
            Possemato R, Chen WW, Sullivan LB, Fiske BP, Cho S, Freinkman E, 
            Birsoy K, Abu-Remaileh M, Shaul YD, Liu CM, Zhou M, Koh MJ, Chung H,
            Davidson SM, Luengo A, Wang AQ, Xu X, Yasgar A, Liu L, Rai G, 
            Westover KD, Vander Heiden MG, Shen M, Gray NS, Boxer MB, Sabatini 
            DM
  TITLE     A PHGDH inhibitor reveals coordination of serine synthesis and 
            one-carbon unit fate.
  JOURNAL   Nat Chem Biol. 2016 Jun;12(6):452-8. doi: 10.1038/nchembio.2070. 
            Epub 2016 Apr 25.
  PUBMED    27110680
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2070"; journalName: "Nat Chem Biol"; date: 
            "2016-06"; volume: "12"; issue: "6"; pages: "452-8"
COMMENT     Sequence Label: pLentiCRISPRv1-sgSHMT1-G3
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(51..98)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tcgccggcct gcttcagcag gctgaagttg gtggcgccgc tgccgctagc tttctttttc
       61 ttagcttgac cagctttctt agtagcagca ggacgcttgt cgcctcccag ctgagacagg
      121 tcgatccgtg tctcgtacag gccggtgatg ctctggtgga tcagggtggc gtccagcacc
      181 tctttggtgc tggtgtacct cttccggtcg atggtggtgt caaagtactt gaaggcggca
      241 ggggctccca gattggtcag ggtaaacagg tggatgatat tctcggcctg ctctctgatg
      301 ggcttatccc ggtgcttgtt gtaggcggac agcactttgt ccagattagc gtcggccagg
      361 atcactctct tggagaactc gctgatctgc tcgatgatct cgtccaggta gtgcttgtgc
      421 tgttccacaa acagctgttt ctgctcatta tcctcggggg agcccttcag cttctcatag
      481 tggctggcca ggtacaggaa gttcacatat ttggagggca gggccagttc gtttcccttc
      541 tgcagttcgc cggcagaggc cagcattctc ttccggccgt tttccagctc gaacagggag
      601 tacttaggca gcttgatgat caggtccttt ttcacttctt tgtagccctt ggcttccaga
      661 aagtcgatgg gattcttctc gaagctgctt ctttccatga tggtgatccc cagcagctct
      721 ttcacactct tcagtttctt ggacttgccc ttttccactt tggccaccac cagcacagaa
      781 taggccacgg tggggctgtc gaagccgccg tacttcttag ggtcccagtc cttctttctg
      841 gcgatcagct tatcgctgtt cctcttgggc aggatagact ctttgctgaa gccgcctgtc
      901 tgcacctcgg tctttttcac gatattcact tggggcatgc tcagcacttt ccgcacggtg
      961 gcaaaatccc ggccctta
//
