LOCUS       sequence-164204-         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of CyRFP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
  TITLE     Simultaneous dual-color fluorescence lifetime imaging with novel 
            red-shifted fluorescent proteins.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
  PUBMED    27798609
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046."
COMMENT     Sequence Label: pNCS-CyRFP1
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             53..75
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             95..112
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             116..148
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(152..160,161..175)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gagtttattc ttgacacctg atgcgatgaa tgatataata ggaaagtact gttttgttta
       61 actttaagaa ggagatatac atatgcgggg ttctcatcat catcatcatc atggtatggc
      121 tagcatgact ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggatcc
      181 gatggtgagc aagggcgagg agctgatcaa ggagaacatg agaagcaagc tgtacctgga
      241 aggcagcgtg aacggccacc agttcaagtg cacccacgaa ggggagggca agccctacga
      301 gggcaagcag accaacagga tcaaggtggt ggagggaggc cccctgccgt tcgcattcga
      361 catcctggcc accatgttta tgtacgggag caaggtgttc atcaagtacc ccgccgacct
      421 ccccgattat tttaagcagt ccttccctga gggcttcaca tgggagagag tcatggtgtt
      481 cgaagacggg ggcgtgctga ccgccaccca ggacaccagc ctccaggacg gcgagctcat
      541 ctacaacgtc aagctcagag gggtgaactt cccagccaac ggccccgtga tgcagaagaa
      601 aacactgggc tgggagccca gcaccgagac catgtacccc gctgacggcg gcctggaagg
      661 cagatgcgac aaggccctga agctcgtggg cgggggccac ctgcacgtca acttc
//
