LOCUS       sequence-164205-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of CyRFP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
  TITLE     Simultaneous dual-color fluorescence lifetime imaging with novel 
            red-shifted fluorescent proteins.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
  PUBMED    27798609
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046."
COMMENT     Sequence Label: pNCS-CyRFP1
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..731)
                     /codon_start=1
                     /product="cyan-excitable red fluorescent protein (Laviv et 
                     al., 2016)"
                     /label=CyRFP1
                     /translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTN
                     RIKVVEGGPLPFAFDILATMFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG
                     VLTATQDTSLQDGELIYNVKLRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCD
                     KALKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY
                     SNLGGGMDELYK"
     CDS             complement(join(738..752,753..761))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(765..797)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(801..818)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             838..860
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 agcagccgga tcaagcttcg aattcattac ttgtacagct cgtccatgcc tccgcccagg
       61 ttggagtatc tggccacagc gtgctcgtac tgctcgacgt aggtctcgtt gtcggcctcc
      121 ttgattcttt ccagtctgcg gtccacgtag tggacgccgg gcatcttcac gggtttcttg
      181 gacttgtatg tggtcttgaa gttgacgtgc aggtggcccc cgcccacgag cttcagggcc
      241 ttgtcgcatc tgccttccag gccgccgtca gcggggtaca tggtctcggt gctgggctcc
      301 cagcccagtg ttttcttctg catcacgggg ccgttggctg ggaagttcac ccctctgagc
      361 ttgacgttgt agatgagctc gccgtcctgg aggctggtgt cctgggtggc ggtcagcacg
      421 cccccgtctt cgaacaccat gactctctcc catgtgaagc cctcagggaa ggactgctta
      481 aaataatcgg ggaggtcggc ggggtacttg atgaacacct tgctcccgta cataaacatg
      541 gtggccagga tgtcgaatgc gaacggcagg gggcctccct ccaccacctt gatcctgttg
      601 gtctgcttgc cctcgtaggg cttgccctcc ccttcgtggg tgcacttgaa ctggtggccg
      661 ttcacgctgc cttccaggta cagcttgctt ctcatgttct ccttgatcag ctcctcgccc
      721 ttgctcacca tcggatcctt atcgtcatcg tcgtacagat cccgacccat ttgctgtcca
      781 ccagtcatgc tagccatacc atgatgatga tgatgatgag aaccccgcat atgtatatct
      841 ccttcttaaa gttaaacaaa
//
