LOCUS       sequence-164210-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pHis-par2-UbHs/Ubc5Sc/ Uba1Ta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pGEN6-Ubc5 Sc
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             127..354
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac atatgtcgta ctaccatcac
       61 catcaccatc acgattacga tatcccaacg accgaaaacc tgtattttca gggcgccatg
      121 ggatccatgc agatcttcgt gaagactctg actggtaaga ccatcaccct cgaggtggag
      181 cccagtgaca ccatcgagaa tgtcaaggca aagatccaag ataaggaagg cattcctcct
      241 gatcagcaga ggttgatctt tgccggaaaa cagctggaag atggtcgtac cctgtctgac
      301 tacaacatcc agaaagagtc caccttgcac ctggtactcc gtctcagagg tgggtgagaa
      361 ttctaaggaa atccattatg tcttcctcca agcgtattgc caaggaatta agtgatttag
      421 ggagagatcc tcctgcttca tgttcagcag gacctgtagg ggatgacctg tatcattggc
      481 aagcctctat tatgggtcct tcagactcac cctacgctgg tggcgttttc tttttgtcta
      541 ttcactttcc aactgattat ccattcaagc caccgaaggt aaactttacg accaaaattt
      601 atcatccgaa tattaattcg agtggtaata tctgccttga tattttaaag gaccagtggt
      661 caccggcgct aaccctttca aaagttttgt tgtctatttg ctctctttta acagatgcta
      721 atcccgacga tcctttggtc cctgaaattg ctcaaatcta caagacagat aaggctaagt
      781 atgaagccac cgctaaggag tggactaaaa aatatgctgt ttgaggcgcg ccacaattcc
      841 gacaggaaac agctatgctt ccccggaagc gggagatcgt cgccggcgag gtcgaggact
      901 tgcagaagaa gacccgcgcc ggggaggggg aggtcactag ggaggaagga gacgcagcc
//
