LOCUS       sequence-164211-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mCyRFP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R
  TITLE     Simultaneous dual-color fluorescence lifetime imaging with novel 
            red-shifted fluorescent proteins.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
  PUBMED    27798609
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046."
COMMENT     Sequence Label: pNCS-mCyRFP1
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             48..70
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             90..107
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             111..143
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(147..155,156..170)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             177..878
                     /codon_start=1
                     /product="monomeric cyan-excitable red fluorescent protein 
                     (Laviv et al., 2016)"
                     /label=mCyRFP1
                     /translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTA
                     RIKVVEGGPLPFAFDILATMFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG
                     VLTATQDTSLQDGELIYNVKLRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCD
                     KKLKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY
                     SNLGGGMDELYK"
ORIGIN
        1 tattcttgac acctgatgcg atgaatgata taataggaaa gtactgtttt gtttaacttt
       61 aagaaggaga tatacatatg cggggttctc atcatcatca tcatcatggt atggctagca
      121 tgactggtgg acagcaaatg ggtcgggatc tgtacgacga tgacgataag gatccgatgg
      181 tgagcaaggg cgaggagctg atcaaggaga acatgagaag caagctgtac ctggaaggca
      241 gcgtgaacgg ccaccagttc aagtgcaccc acgaagggga gggcaagccc tacgagggca
      301 agcagaccgc gaggatcaag gtggtggagg gaggccccct gccgttcgca ttcgacatcc
      361 tggccaccat gtttatgtac gggagcaagg tgttcatcaa gtaccccgcc gacctccccg
      421 attattttaa gcagtccttc cctgagggct tcacatggga gagagtcatg gtgttcgaag
      481 acgggggcgt gctgaccgcc acccaggaca ccagcctcca ggacggcgag ctcatctaca
      541 acgtcaagct cagaggggtg aacttcccag ccaacggccc cgtgatgcag aagaaaacac
      601 tgggctggga gcccagcacc gagaccatgt accccgctga cggcggcctg gaaggcagat
      661 gcgacaagaa gctgaagctc gtgggcgggg gccacctgca cgtcaacttc aagaccacat
      721 acaagtccaa gaaacccgtg aagatgcccg gcgtccacta cgtggaccgc agactggaaa
      781 gaatcaagga ggccgacaac gagacctacg tcgagcagta cgagcacgct gtggccagat
      841 actccaacct gggcggaggc atggacgagc tgtacaagta atgaattcga agcttgatcc
      901 ggctgctaac
//
