LOCUS       sequence-164222-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN 
            nuclease 2 inactive mutant)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian
            R, Doudna JA
  TITLE     Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA 
            processing and RNA detection.
  JOURNAL   Nature. 2016 Sep 26. doi: 10.1038/nature19802.
  PUBMED    27669025
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 26. doi: 10.1038/nature19802."
COMMENT     Sequence Label: p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..48
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 aacaataaca acaacctcgg gatcgaggaa aacctgtact tccaatccaa tgcaatgaaa
       61 gtgacgaagg taggaggcat ttcgcataag aagtacacgt ccgaaggccg cttagtgaag
      121 tcagaatcgg aagaaaatcg cacagacgaa cgtctgtcgg cgttgcttaa tatgcgcctt
      181 gacatgtata tcaagaatcc cagcagcacg gaaaccaagg aaaatcaaaa acgcattggg
      241 aaattaaaga aattcttctc aaacaaaatg gtctatctta aagacaatac cttgagtttg
      301 aagaatggga aaaaggagaa cattgatcgt gagtattctg agactgacat ccttgagagc
      361 gatgtccgtg acaagaaaaa cttcgccgtg ttgaaaaaga tctatctgaa tgaaaacgtg
      421 aactcggagg aattggaagt ttttcgtaac gacattaaga agaaactgaa caaaatcaac
      481 agcctgaagt actcatttga aaagaataag gcgaattatc aaaagattaa tgagaataac
      541 atcgagaagg ttgaaggtaa gtcaaagcgt aacattattt acgattatta tcgtgagtca
      601 gcgaaacgtg acgcttatgt aagcaatgtg aaagaagcct ttgataagct ttacaaggaa
      661 gaggacattg caaaacttgt tcttgaaatt gagaacctta cgaagttaga gaaatacaag
      721 attcgcgagt tctaccacga aattattgga cgtaagaatg acaaggaaaa ctttgcaaaa
      781 atcatctacg aagaaatcca gaatgttaat aacatgaaag agttgatcga ga
//
