LOCUS       sequence-164224-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN 
            nuclease 2 inactive mutant)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian
            R, Doudna JA
  TITLE     Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA 
            processing and RNA detection.
  JOURNAL   Nature. 2016 Sep 26. doi: 10.1038/nature19802.
  PUBMED    27669025
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 26. doi: 10.1038/nature19802."
COMMENT     Sequence Label: p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttgttagcag ccggatcagc gcgcacgcgt ggtaccggcc ggccaccagg tggcgcgccg
       61 cgatcgcgga tccgttatcc acttccaatg ttattagttt tcagactttt tctcttccat
      121 tttgtattca aacataatct tcaccaattt gcaaagttcc tcggaattgc ggtcagtcat
      181 taacttcttc ttcttaagat tcttaagatg cacgattttt tcgctctcca gtgtctgaat
      241 accgatcttc ttgtccgccc cgattttgaa tgtggctaca aaaccatact ccttaaggat
      301 atcaactact gatttcatta cggcattttt cagtttgcga tcgtaggaca gcaatttacg
      361 caaattttca aggacttcca gcagtgagat ctcggcgtga ggaatataat tgaatgcggc
      421 gatgtaatta gcaatataaa ggtccttctt ttcttgcttc aacaccttga tattcgcgga
      481 actgtacttg ttgatcttaa cttcatcatt ttgatgtaat tccttgtaga atttaatgta
      541 tttctctaca atctgacccc ctttgtactt cacgttcttc ttattttcaa agttgaagat
      601 ctcttcgatg tattggtttt ctgggaactc acctttaagg cggaagcgca gatcgcgttc
      661 ccaaattgag gtatatccga ctaaacgatg taaaatgcgc agcaacaggc cctgcagtaa
      721 attcagctca ttgaattcta ccttgttctt aaggtgggtg tactcctcaa tattctcaat
      781 agcttgcttg taactttcat agtcctcatc tgtaaacttc tcgtctttac ggggacgagc
      841 gtatttgcgg tgcagatttt cctgcatctt atggttcttc tcgatctcat tctttttatt
      901 ggagtatttc ttcagctctt cgatcgagat cttgtatcca gccttgtcgg caattt
//
