LOCUS       sequence-164225-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of L. buccalis C2c2 CRISPR effector (double 
            HEPN nuclease 1 and 2 inactive mutant)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian
            R, Doudna JA
  TITLE     Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA 
            processing and RNA detection.
  JOURNAL   Nature. 2016 Sep 26. doi: 10.1038/nature19802.
  PUBMED    27669025
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 26. doi: 10.1038/nature19802."
COMMENT     Sequence Label: p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..49
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 taacaataac aacaacctcg ggatcgagga aaacctgtac ttccaatcca atgcaatgaa
       61 agtgacgaag gtaggaggca tttcgcataa gaagtacacg tccgaaggcc gcttagtgaa
      121 gtcagaatcg gaagaaaatc gcacagacga acgtctgtcg gcgttgctta atatgcgcct
      181 tgacatgtat atcaagaatc ccagcagcac ggaaaccaag gaaaatcaaa aacgcattgg
      241 gaaattaaag aaattcttct caaacaaaat ggtctatctt aaagacaata ccttgagttt
      301 gaagaatggg aaaaaggaga acattgatcg tgagtattct gagactgaca tccttgagag
      361 cgatgtccgt gacaagaaaa acttcgccgt gttgaaaaag atctatctga atgaaaacgt
      421 gaactcggag gaattggaag tttttcgtaa cgacattaag aagaaactga acaaaatcaa
      481 cagcctgaag tactcatttg aaaagaataa ggcgaattat caaaagatta atgagaataa
      541 catcgagaag gttgaaggta agtcaaagcg taacattatt tacgattatt atcgtgagtc
      601 agcgaaacgt gacgcttatg taagcaatgt gaaagaagcc tttgataagc tttacaagga
      661 agaggacatt gcaaaacttg ttcttgaaat tgagaacctt acgaagttag agaaatacaa
      721 gattcgcgag ttctaccacg aaattattgg acgtaagaat gacaaggaaa actttgcaaa
      781 aatcatctac gaagaaatcc agaatgttaa taacatgaaa gagttgatcg aga
//
