LOCUS       sequence-164226-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of L. buccalis C2c2 CRISPR effector (double 
            HEPN nuclease 1 and 2 inactive mutant)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian
            R, Doudna JA
  TITLE     Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA 
            processing and RNA detection.
  JOURNAL   Nature. 2016 Sep 26. doi: 10.1038/nature19802.
  PUBMED    27669025
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 26. doi: 10.1038/nature19802."
COMMENT     Sequence Label: p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gttagcagcc ggatcagcgc gcacgcgtgg taccggccgg ccaccaggtg gcgcgccgcg
       61 atcgcggatc cgttatccac ttccaatgtt attagttttc agactttttc tcttccattt
      121 tgtattcaaa cataatcttc accaatttgc aaagttcctc ggaattgcgg tcagtcatta
      181 acttcttctt cttaagattc ttaagatgca cgattttttc gctctccagt gtctgaatac
      241 cgatcttctt gtccgccccg attttgaatg tggctacaaa accatactcc ttaaggatat
      301 caactactga tttcattacg gcatttttca gtttgcgatc gtaggacagc aatttacgca
      361 aattttcaag gacttccagc agtgagatct cggcgtgagg aatataattg aatgcggcga
      421 tgtaattagc aatataaagg tccttctttt cttgcttcaa caccttgata ttcgcggaac
      481 tgtacttgtt gatcttaact tcatcatttt gatgtaattc cttgtagaat ttaatgtatt
      541 tctctacaat ctgaccccct ttgtacttca cgttcttctt attttcaaag ttgaagatct
      601 cttcgatgta ttggttttct gggaactcac ctttaaggcg gaagcgcaga tcgcgttccc
      661 aaattgaggt atatccgact aaacgatgta aaatgcgcag caacaggccc tgcagtaaat
      721 tcagctcatt gaattctacc ttgttcttaa ggtgggtgta ctcctcaata ttctcaatag
      781 cttgcttgta actttcatag tcctcatctg taaacttctc gtctttacgg ggacgagcgt
      841 atttgcggtg cagattttcc tgcatcttat ggttcttctc gatctcattc tttttattgg
      901 agtatttctt cagctcttcg atcgagatct tgtatccagc cttgtcggca att
//
