LOCUS       sequence-164227-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of L. buccalis C2c2 CRISPR effector (double 
            HEPN nuclease 1 and 2 inactive mutant)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian
            R, Doudna JA
  TITLE     Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA 
            processing and RNA detection.
  JOURNAL   Nature. 2016 Sep 26. doi: 10.1038/nature19802.
  PUBMED    27669025
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 26. doi: 10.1038/nature19802."
COMMENT     Sequence Label: p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 attactgggc gtatgcgcgg caaaacagtt aagaacaata aaggtgaaga gaagtacgtg
       61 tccggagaag ttgataagat ctataatgaa aataagaaga acgaggttaa ggagaactta
      121 aaaatgttct attcgtacga tttcaatatg gacaacaaga atgaaatcga agatttcttc
      181 gccaacatcg acgaggcgat ttcttccatc gctcacggta ttgtcgcctt caacttggaa
      241 ttagaaggta aggatatctt tgcgttcaag aacattgcgc catccgaaat ctcaaagaag
      301 atgtttcaga atgagattaa cgagaaaaaa ctgaaattga agatctttcg tcaactgaac
      361 tctgccaacg tgttccgcta tctcgaaaag tataaaattc tgaattacct taaacgtaca
      421 cgcttcgagt ttgtcaataa aaatatccca ttcgtcccgt ctttcaccaa attatattcg
      481 cgcattgatg acctgaagaa tagtcttggg atttactgga aaactccgaa aacaaacgac
      541 gacaataaga ctaaggagat tattgatgcc caaatctatt tgcttaaaaa catctattac
      601 ggggagttcc tgaattattt catgtcgaac aatggtaatt tctttgagat ttctaaagaa
      661 atcatcgaat tgaacaagaa cgataaacgc aacttaaaga ctgggtttta caagctgcaa
      721 aagtttgaag acatccagga gaagattcca aaggaatact tggcgaatat ccagtccctg
      781 tacatgatta atgccggtaa tc
//
