LOCUS       sequence-164229-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bidirectional tetracycline-regulated viral reporter that measures 
            the effects of 3' UTR sequences on mRNA and protein production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Zhao W, Pollack JL, Blagev DP, Zaitlen N, McManus MT, Erle DJ
  TITLE     Massively parallel functional annotation of 3' untranslated regions.
  JOURNAL   Nat Biotechnol. 2014 Apr;32(4):387-91. doi: 10.1038/nbt.2851. Epub 
            2014 Mar 16.
  PUBMED    24633241
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2851"; 
            journalName: "Nat Biotechnol"; date: "2014-04"; volume: "32"; issue:
            "4"; pages: "387-91"
COMMENT     Sequence Label: BTV
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    311..445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     389..408
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    582..699
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tctcggcatg gacgagctgt acaagtagcg gccggccgcg tcgacctagt tctagctagc
       61 aactacgcgt agtcgatatc cttacctgca gggcctttaa ttaaactggc tagcttagtc
      121 atgcaccggt ggatccagac cacctcccct gcgagctaag ctggacagcc aatgacgggt
      181 aagagagtga catttttcac taacctaaga caggagggcc gtcagagcta ctgcctaatc
      241 caaagacggg taaaagtgat aaaaatgtat cactccaacc taagacaggc gcagcttccg
      301 agggatttga gatccagaca tgataagata cattgatgag tttggacaaa ccaaaactag
      361 aatgcagtga aaaaaatgcc ttatttgtga aatttgtgat gctattgcct tatttgtaac
      421 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
      481 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
      541 tgattatgat cagatctctc gaggctagtc tcgtgatcga taaaattttg aatttttgta
      601 atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc
      661 cctgcactgt accccccaat cccccctttt cttttaaaag ttaaccgata ccgtcgagat
      721 ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct
      781 tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat
      841 ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag
      901 cctcctacta tcattatga
//
