LOCUS       sequence-164230-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of WT L. seeligeri C2c2 CRISPR effector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian
            R, Doudna JA
  TITLE     Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA 
            processing and RNA detection.
  JOURNAL   Nature. 2016 Sep 26. doi: 10.1038/nature19802.
  PUBMED    27669025
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 26. doi: 10.1038/nature19802."
COMMENT     Sequence Label: p2CT-His-MBP-Lse_C2c2_WT
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..49
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 taacaataac aacaacctcg ggatcgagga aaacctgtac ttccaatcca atgcaatgtg
       61 gatcagcatc aagaccttaa tccatcacct tggggtcctg ttcttctgcg actatatgta
      121 caatcgccgt gagaagaaaa ttattgaagt caagacgatg cgtattacca aagtagaagt
      181 ggatcgcaaa aaggtgctta tctcccgcga taagaatggt ggaaaactgg tttacgaaaa
      241 tgagatgcag gataatacgg agcagatcat gcaccacaaa aagtcctcat tttacaagtc
      301 tgtcgtcaat aaaacaattt gccgtccaga gcaaaaacaa atgaaaaagt tagtgcatgg
      361 gcttttacag gaaaactctc aagagaagat caaagtatcg gatgttacca agcttaacat
      421 ctcgaacttt cttaatcatc gcttcaaaaa aagcctttac tacttcccgg aaaattctcc
      481 ggataaaagc gaggagtacc gcattgagat taatttaagt cagttgcttg aagattcgct
      541 gaagaagcag caggggacct ttatttgctg ggagagcttt tcaaaagaca tggagctgta
      601 cattaattgg gccgaaaatt atatttcgag caaaaccaaa cttattaaga aatctattcg
      661 taataatcgt atccagtcta cggaaagccg ttccggacag ttaatggatc gctacatgaa
      721 agacatcctg aacaagaata agccttttga cattcagtca gtctcggaaa agtaccagtt
      781 ggaaaaatta acttcggcac tgaaggctac ctttaaggag gctaagaaga atgacaagga
      841 gattaactat aaattaaaga gcacgctgca gaaccatgaa cgccagatca tcgaggaatt
      901 aaaagagaac tccgaactta accaattcaa catcgaaatc cgtaagcatc ttgagaccta
      961 cttcc
//
