LOCUS       sequence-164239-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of WT L. shahii C2c2 CRISPR effector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian
            R, Doudna JA
  TITLE     Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA 
            processing and RNA detection.
  JOURNAL   Nature. 2016 Sep 26. doi: 10.1038/nature19802.
  PUBMED    27669025
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 26. doi: 10.1038/nature19802."
COMMENT     Sequence Label: p2CT-His-MBP-Lsh_C2c2_WT
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..49
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 taacaataac aacaacctcg ggatcgagga aaacctgtac ttccaatcca atgcaatggg
       61 gaaccttttc gggcataaac gctggtacga ggtgcgcgat aagaaggact ttaagatcaa
      121 acgcaaagtc aaggttaagc gtaattacga cggtaacaaa tacatcttaa acatcaacga
      181 gaacaacaat aaggagaaaa tcgacaataa caagtttatt cgtaagtaca tcaattacaa
      241 gaaaaacgac aatatcctta aagaatttac gcgcaaattc catgcaggca atatcttgtt
      301 caagttaaag ggtaaggagg ggatcattcg tatcgagaac aatgatgact ttctggaaac
      361 tgaagaggtt gtcctttaca tcgaagcgta tgggaagagc gagaagctga aagctctggg
      421 aatcactaaa aaaaaaatca ttgacgaggc gattcgtcaa ggcatcacaa aggatgacaa
      481 gaaaatcgaa atcaagcgcc aagagaacga agaagaaatc gaaatcgaca tccgcgatga
      541 atacaccaac aagaccttaa atgattgcag tattattctg cgcatcattg aaaacgatga
      601 gcttgagacg aaaaagtcta tctatgagat ctttaagaat atcaacatgt cgttgtacaa
      661 gattatcgag aagattattg aaaatgagac tgagaaagtc tttgaaaacc gttattatga
      721 agagcacctt cgcgaaaaac tgcttaagga cgacaaaatt gatgttattc tgactaactt
      781 tatggagatt cgtgagaaga tcaagagcaa cctggaaatt ttagggttcg tcaaattcta
      841 cttaaacgta gg
//
