LOCUS       sequence-164249-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed 
            as an infection/transfection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Tang JC, Drokhlyansky E, Etemad B, Rudolph S, Guo B, Wang S, Ellis 
            EG, Li JZ, Cepko CL
  TITLE     Detection and manipulation of live antigen-expressing cells using 
            conditionally stable nanobodies.
  JOURNAL   Elife. 2016 May 20;5. pii: e15312. doi: 10.7554/eLife.15312.
  PUBMED    27205882
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-05-20"; volume: "5. pii: e15312. doi"; pages: " 
            10.7554/eLife.15312"
COMMENT     Sequence Label: pBMN-dGBP1-TagBFP
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             153..836
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
ORIGIN
        1 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
       61 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
      121 ttgaaaaaca cgatgataat atggccacaa ccatggtgag cggcctgctg aaggagagta
      181 tgcgcatcaa gatgtacatg gagggcaccg tgaacggcca ctacttcaag tgcgagggcg
      241 agggcgacgg caaccccttc gccggcaccc agagcatgag aatccacgtg accgagggcg
      301 cccccctgcc cttcgccttc gacatcctgg ccccctgctg cgagtacggc agcaggacct
      361 tcgtgcacca caccgccgag atccccgact tcttcaagca gagcttcccc gagggcttca
      421 cctgggagag aaccaccacc tacgaggacg gcggcatcct gaccgcccac caggacacca
      481 gcctggaggg caactgcctg atctacaagg tgaaggtgca cggcaccaac ttccccgccg
      541 acggccccgt gatgaagaac aagagcggcg gctgggagcc cagcaccgag gtggtgtacc
      601 ccgagaacgg cgtgctgtgc ggccggaacg tgatggccct gaaggtgggc gaccggcacc
      661 tgatctgcca ccactacacc agctaccgga gcaagaaggc cgtgcgcgcc ctgaccatgc
      721 ccggcttcca cttcaccgac atccggctcc agatgctgcg gaagaagaag gacgagtact
      781 tcgagctgta cgaggccagc gtggcccggt acagcgacct gcccgagaag gccaacagat
      841 ctcccgggat ggtgagcggc ctgctgaagg agagtatgcg catcaagatg tacatggagg
      901 gcaccgtgaa cggccactac ttcaagtgcg agggcgaggg cgacggcaac cccttcgccg
      961 gcacccagag catgagaatc cacgtgaccg agggcgcccc c
//
