LOCUS       sequence-164252-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of doxycycline-inducible dominant negative 
            Rac1 and co-expression of Venus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   MacKay JL, Kumar S
  TITLE     Simultaneous and independent tuning of RhoA and Rac1 activity with 
            orthogonally inducible promoters.
  JOURNAL   Integr Biol (Camb). 2014 Sep;6(9):885-94. doi: 10.1039/c4ib00099d.
  PUBMED    25044255
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Integr Biol (Camb)"; date: "2014-09"; volume: "6"; issue: "9"; 
            pages: "885-94"
COMMENT     Sequence Label: pSLIK DN Rac1
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    77..101
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        133..447
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    141..159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    177..195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    212..230
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    248..266
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    284..302
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    319..337
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    355..373
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     420..444
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa
       61 ttctagacag ttatcaacaa gtttgtacaa aaaagcaggc tttaaaggaa ccaattcagt
      121 cgactggatc ccgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc
      181 tatcagtgat agagaacgat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      241 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
      301 gaacgtatgt cgagtttatc cctatcagtg atagagaacg tatgtcgagt ttactcccta
      361 tcagtgatag agaacgtatg tcgaggtagg cgtgtacggt gggaggccta tataagcaga
      421 gctcgtttag tgaaccgtca gatcgccact agtcgactgg atccggtacc gaattcatgc
      481 aggccatcaa gtgtgtggtg gtgggagacg gagctgtagg taaaaactgc ctactgatca
      541 gttacacaac caatgcattt cctggagaat atatccctac tgtctttgac aattattctg
      601 ccaatgttat ggtagatgga aaaccggtga atctgggctt atgggataca gctggacaag
      661 aagattatga cagattacgc cccctatcct atccgcaaac agatgtgttc ttaatttgct
      721 tttcccttgt gagtcctgca tcatttgaaa atgtccgtgc aaagtggtat cctgaggtgc
      781 ggcaccactg tcccaacact cccatcat
//
