LOCUS       sequence-164253-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Avi-tagged RPL1 and humanized birA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ingolia NT, Brar GA, Stern-Ginossar N, Harris MS, Talhouarne GJ, 
            Jackson SE, Wills MR, Weissman JS
  TITLE     Ribosome profiling reveals pervasive translation outside of 
            annotated protein-coding genes.
  JOURNAL   Cell Rep. 2014 Sep 11;8(5):1365-79. doi: 
            10.1016/j.celrep.2014.07.045. Epub 2014 Aug 21.
  PUBMED    25159147
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.07.045"; journalName: "Cell Rep"; date: 
            "2014-09-11- 11"; volume: "8"; issue: "5"; pages: "1365-79"
COMMENT     Sequence Label: pNTI194
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             775..795
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             793..837
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             847..903
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc
       61 atgagcagca aagtctctcg cgacaccctg tacgaggcgg tgcgggaagt cctgcacggg
      121 aaccagcgca agcgccgcaa gttcctggag acggtggagt tgcagatcag cttgaagaac
      181 tatgatcccc agaaggacaa gcgcttctcg ggcaccgtca ggcttaagtc cactccccgc
      241 cctaagttct ctgtgtgtgt cctgggggac cagcagcact gtgacgaggc taaggccgtg
      301 gatatccccc acatggacat cgaggcgctg aaaaaactca acaagaataa aaaactggtc
      361 aagaagctgg ccaagaagta tgatgcgttt ttggcctcag agtctctgat caagcagatt
      421 ccacgaatcc tcggcccagg tttaaataag gcaggaaagt tcccttccct gctcacacac
      481 aacgaaaaca tggtggccaa agtggatgag gtgaagtcca caatcaagtt ccaaatgaag
      541 aaggtgttat gtctggctgt agctgttggt cacgtgaaga tgacagacga tgagcttgtg
      601 tataacattc acctggctgt caacttcttg gtgtcattgc tcaagaaaaa ctggcagaat
      661 gtccgggcct tatatatcaa gagcaccatg ggcaagcccc agcgcctata tggaggttct
      721 tcaggaagtg ggagttcagg aggctccgga tcaagcggca gtagtggtgg aagcgaaaac
      781 ctgtacttcc agggcctgaa tgacatcttt gaggcccaga agattgaatg gcacgaggga
      841 agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga
      901 cctatgaagg acaacaccgt gcccctgaag ctgatcgccc tgctggccaa tggcgagttc
      961 cactctggcg agcagctggg cgaaaccctg ggaatgtctg ccgaggccat caaca
//
