LOCUS       sequence-164257-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Avi-tagged RPL1 and humanized birA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ingolia NT, Brar GA, Stern-Ginossar N, Harris MS, Talhouarne GJ, 
            Jackson SE, Wills MR, Weissman JS
  TITLE     Ribosome profiling reveals pervasive translation outside of 
            annotated protein-coding genes.
  JOURNAL   Cell Rep. 2014 Sep 11;8(5):1365-79. doi: 
            10.1016/j.celrep.2014.07.045. Epub 2014 Aug 21.
  PUBMED    25159147
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.07.045"; journalName: "Cell Rep"; date: 
            "2014-09-11- 11"; volume: "8"; issue: "5"; pages: "1365-79"
COMMENT     Sequence Label: pNTI194
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..55)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggtttaaacg ggccctctag actcgagctc acttatcgtc atcgtccttg tagtccttct
       61 cggcagatct caggctgatc tcgccgccca tccagggctt gatgatgccg tcctgttcca
      121 gcagcagagc gccctgcttg tcgatgcccc ggctgatgcc gaagatctct ttgtcgccga
      181 tgatcagttt cacgggtctg ttgatgaagt tgtccagctt ctcccatctg gacaggtagg
      241 gggtcaggcc ttcctgctcg aacagttcca gggcggctct cagctctctg atcagcatgg
      301 cggccagggt gtttctgtcc aggttgattc cggcttcctg cagggtgatc cagccctgat
      361 tcacgacgga ctcttccacc cgccgcatgg ccatattgat gccggctcca atcacgatct
      421 gggcggcgtc gcctgttttg cctgtcagtt ccaccaggat gccggccagc ttccggtcct
      481 gcagatacag gtcgttgggc cacttcacgc gcactttgtc ggcgcccagc tttctcagca
      541 cctcggccat cacgatgccg atcacgaggc tcagtccaat agcggcggca ggtccctgtt
      601 ccagccgcca gaacatgctc aggtacagat tggcgccgaa ggggctgaac cacttgcggc
      661 ctcttctccc ccttccggcg tgctgatact cggccacgca ggcatctccg cttttcagct
      721 cgccgatccg gtccagcagg tactggttgg tgctgtcgat cacgggcagc actgccacgc
      781 tgccatcgtc cagctggctc aggatcttct cggcgttcag cagctggata ggctcgggca
      841 ggctgtagcc cttgccaggc actgtgaaca cgtccacgcc ccaatcccgc agggtctgga
      901 tgtgcttgtt gatggcctcg gcagacattc ccagggtttc gcccagctgc tcgccagagt
      961 ggaactcgcc attggccagc agggcgatca gcttcagggg cacggtgttg tccttcatag
//
