LOCUS       sequence-164261-        4887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing BRCA1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_BRCA1_WT
FEATURES             Location/Qualifiers
     source          1..4887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2287..2386)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2403..2422)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2404..2422)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2427..2443)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2427..2443)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2874..3665
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3758..4346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4247..4266
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4500..4517
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4676..4703)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4795..4881)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat ggatttatct gctcttcgcg ttgaagaagt acaaaatgtc attaatgcta
      241 tgcagaaaat cttagagtgt cccatctgtc tggagttgat caaggaacct gtctccacaa
      301 agtgtgacca catattttgc aaattttgca tgctgaaact tctcaaccag aagaaagggc
      361 cttcacagtg tcctttatgt aagaatgata taaccaaaag gagcctacaa gaaagtacga
      421 gatttagtca acttgttgaa gagctattga aaatcatttg tgcttttcag cttgacacag
      481 gtttggagta tgcaaacagc tataattttg caaaaaagga aaataactct cctgaacatc
      541 taaaagatga agtttctatc atccaaagta tgggctacag aaaccgtgcc aaaagacttc
      601 tacagagtga acccgaaaat ccttccttgc aggaaaccag tctcagtgtc caactctcta
      661 accttggaac tgtgagaact ctgaggacaa agcagcggat acaacctcaa aagacgtctg
      721 tctacattga attgggatct gattcttctg aagataccgt taataaggca acttattgca
      781 gtgtgggaga tcaagaattg ttacaaatca cccctcaagg aaccagggat gaaatcagtt
      841 tggattctgc aaaaaaggct gcttgtgaat tttctgagac ggatgtaaca aatactgaac
      901 atcatcaacc cagtaataat gatttgaaca ccactgagaa gcgtgcagct gagaggcatc
      961 cagaaaagta tcagggtgaa gcagcatctg ggtgtgagag tgaaacaagc gtctctgaag
     1021 actgctcagg gctatcctct cagagtgaca ttttaaccac tcagcagagg gataccatgc
     1081 aacataacct gataaagctc cagcaggaaa tggctgaact agaagctgtg ttagaacagc
     1141 atgggagcca gccttctaac agctaccctt ccatcataag tgactcttct gcccttgagg
     1201 acctgcgaaa tccagaacaa agcacatcag aaaaagtatt aacttcacag aaaagtagtg
     1261 aataccctat aagccagaat ccagaaggcc tttctgctga caagtttgag gtgtctgcag
     1321 atagttctac cagtaaaaat aaagaaccag gagtggaaag gtcatcccct tctaaatgcc
     1381 catcattaga tgataggtgg tacatgcaca gttgctctgg gagtcttcag aatagaaact
     1441 acccatctca agaggagctc attaaggttg ttgatgtgga ggagcaacag ctggaagagt
     1501 ctgggccaca cgatttgacg gaaacatctt acttgccaag gcaagatcta gagggaaccc
     1561 cttacctgga atctggaatc agcctcttct ctgatgaccc tgaatctgat ccttctgaag
     1621 acagagcccc agagtcagct cgtgttggca acataccatc ttcaacctct gcattgaaag
     1681 ttccccaatt gaaagttgca gaatctgccc agagtccagc tgctgctcat actactgata
     1741 ctgctgggta taatgcaatg gaagaaagtg tgagcaggga gaagccagaa ttgacagctt
     1801 caacagaaag ggtcaacaaa agaatgtcca tggtggtgtc tggcctgacc ccagaagaat
     1861 ttatgctcgt gtacaagttt gccagaaaac accacatcac tttaactaat ctaattactg
     1921 aagagactac tcatgttgtt atgaaaacag atgctgagtt tgtgtgtgaa cggacactga
     1981 aatattttct aggaattgcg ggaggaaaat gggtagttag ctatttctgg gtgacccagt
     2041 ctattaaaga aagaaaaatg ctgaatgagc atgattttga agtcagagga gatgtggtca
     2101 atggaagaaa ccaccaaggt ccaaagcgag caagagaatc ccaggacaga aagatcttca
     2161 gggggctaga aatctgttgc tatgggccct tcaccaacat gcccacaggg tgtccaccca
     2221 attgtggttg tgcagccaga tgcctggaca gaggacaatg gcttccatgc aattgggcag
     2281 atgtgtgccc aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     2341 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     2401 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     2461 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga
     2521 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     2581 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     2641 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     2701 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     2761 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     2821 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     2881 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     2941 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     3001 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     3061 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     3121 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     3181 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     3241 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     3301 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     3361 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     3421 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     3481 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     3541 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     3601 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     3661 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     3721 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3781 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3841 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3901 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3961 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4021 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4081 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4141 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4201 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4261 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4321 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4381 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4441 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4501 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4561 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4621 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     4681 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     4741 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     4801 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     4861 ctgagccttt cgttttattt gatgcct
//
