LOCUS       sequence-164263-        4770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing BTK , part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_BTK_WT
FEATURES             Location/Qualifiers
     source          1..4770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             152..943
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1036..1624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1525..1544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1778..1795
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1954..1981)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2073..2159)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2208..2230
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2222..2239
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2223..2239
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2255..2354
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4335..4434)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4451..4470)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4452..4470)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4475..4491)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4475..4491)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca
       61 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
      121 gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg ccgaagtatc
      181 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
      241 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
      301 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
      361 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
      421 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
      481 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
      541 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
      601 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
      661 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
      721 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
      781 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
      841 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
      901 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga gccacccatg
      961 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     1021 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1081 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1141 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1201 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1261 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1321 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1381 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1441 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1501 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1561 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1621 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1681 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1741 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1801 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1861 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg
     1921 taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc
     1981 tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt
     2041 tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc
     2101 gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga
     2161 tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg
     2221 ttgtaaaacg acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat
     2281 agtgacctgt tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta
     2341 caaaaaagtt ggcaccatgg ccgcagtgat tctggagagc atctttctga agcgatccca
     2401 acagaaaaag aaaacatcac ctctaaactt caagaagcgc ctgtttctct tgaccgtgca
     2461 caaactctcc tactatgagt atgactttga acgtgggaga agaggcagta agaagggttc
     2521 aatagatgtt gagaagatca cttgtgttga aacagtggtt cctgaaaaaa atcctcctcc
     2581 agaaagacag attccgagaa gaggtgaaga gtccagtgaa atggagcaaa tttcaatcat
     2641 tgaaaggttc ccttatccct tccaggttgt atatgatgaa gggcctctct acgtcttctc
     2701 cccaactgaa gaactaagga agcggtggat tcaccagctc aaaaacgtaa tccggtacaa
     2761 cagtgatctg gttcagaaat atcacccttg cttctggatc gatgggcagt atctctgctg
     2821 ctctcagaca gccaaaaatg ctatgggctg ccaaattttg gagaacagga atggaagctt
     2881 aaaacctggg agttctcacc ggaagacaaa aaagcctctt cccccaacgc ctgaggagga
     2941 ccagatcttg aaaaagccac taccgcctga gccagcagca gcaccagtct ccacaagtga
     3001 gctgaaaaag gttgtggccc tttatgatta catgccaatg aatgcaaatg atctacagct
     3061 gcggaagggt gatgaatatt ttatcttgga ggaaagcaac ttaccatggt ggagagcacg
     3121 agataaaaat gggcaggaag gctacattcc tagtaactat gtcactgaag cagaagactc
     3181 catagaaatg tatgagtggt attccaaaca catgactcgg agtcaggctg agcaactgct
     3241 aaagcaagag gggaaagaag gaggtttcat tgtcagagac tccagcaaag ctggcaaata
     3301 tacagtgtct gtgtttgcta aatccacagg ggaccctcaa ggggtgatac gtcattatgt
     3361 tgtgtgttcc acacctcaga gccagtatta cctggctgag aagcaccttt tcagcaccat
     3421 ccctgagctc attaactacc atcagcacaa ctctgcagga ctcatatcca ggctcaaata
     3481 tccagtgtct caacaaaaca agaatgcacc ttccactgca ggcctgggat acggatcatg
     3541 ggaaattgat ccaaaggacc tgaccttctt gaaggagctg gggactggac aatttggggt
     3601 agtgaagtat gggaaatgga gaggccagta cgacgtggcc atcaagatga tcaaagaagg
     3661 ctccatgtct gaagatgaat tcattgaaga agccaaagtc atgatgaatc tttcccatga
     3721 gaagctggtg cagttgtatg gcgtctgcac caagcagcgc cccatcttca tcatcactga
     3781 gtacatggcc aatggctgcc tcctgaacta cctgagggag atgcgccacc gcttccagac
     3841 tcagcagctg ctagagatgt gcaaggatgt ctgtgaagcc atggaatacc tggagtcaaa
     3901 gcagttcctt caccgagacc tggcagctcg aaactgtttg gtaaacgatc aaggagttgt
     3961 taaagtatct gatttcggcc tgtccaggta tgtcctggat gatgaataca caagctcagt
     4021 aggctccaaa tttccagtcc ggtggtcccc accggaagtc ctgatgtata gcaagttcag
     4081 cagcaaatct gacatttggg cttttggggt tttgatgtgg gaaatttact ccctggggaa
     4141 gatgccatat gagagattta ctaacagtga gactgctgaa cacattgccc aaggcctacg
     4201 tctctacagg cctcatctgg cttcagagaa ggtatatacc atcatgtaca gttgctggca
     4261 tgagaaagca gatgagcgtc ccactttcaa aattcttctg agcaatattc tagatgtcat
     4321 ggatgaagaa tccttgccaa ctttcttgta caaagttggc attataagaa agcattgctt
     4381 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     4441 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     4501 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct
     4561 cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg
     4621 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg
     4681 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     4741 cagcggtggt aacggcgcag tggcggtttt
//
