LOCUS       sequence-164278-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd1, construct: 125-312, pET28b(+) NC-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, 
            Mobashery S, Kim SJ, Suh SW
  TITLE     Structural Basis of the Heterodimer Formation between Cell 
            Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori.
  JOURNAL   PLoS One. 2016 Oct 6;11(10):e0164243. doi: 
            10.1371/journal.pone.0164243. eCollection 2016.
  PUBMED    27711177
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-10-6"; volume: "11(10):e0164243. doi"; pages: " 
            10.1371/journal.pone.0164243. eCollection 2016"
COMMENT     Sequence Label: csd1_125-312/pET28b(+)
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             678..695
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(748..766)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      762..809
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(933..952)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggtgc cgcgcggcag ccatatggct gggattaccg
      121 ggcttcaaaa aagctttatc atgcgcctta tccctaatga ctacccgcta gaatcctatc
      181 ggcgcgtttc agccgccttt aataaaagaa tccaccctat tttgcatgtg ctgcacaacc
      241 ataccgggct tgatttaagc accgctatta acacccctgt gtatgcgagc gcgagcgggg
      301 tagtagggtt agcgagcaag gggtggaatg gggggtatgg gaatttgatt aaagttttcc
      361 acccttttgg ttttaaaacc tactacgccc atttgaataa aatcgtcgta aaaacgggcg
      421 aatttgtcaa aaaagggcag ttgattgggt atagtggtaa tacagggatg agcacagggc
      481 cgcatttgca ttatgaagtg cgttttttag atcaacccat aaaccccatg agtttcacca
      541 aatggaacat gaaagatttt gaagaagttt tcaataaaga aaggagcatc agatggcaat
      601 ctttgataac aataataaat cggctaatgc aaaaacagga ccagcgacta tcatcgctca
      661 aggcacaaaa actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
      721 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
      781 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga
      841 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      901 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      961 cgccacgttc gccggcttt
//
