LOCUS       sequence-164279-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd1, construct: 125-312, pET28b(+) NC-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, 
            Mobashery S, Kim SJ, Suh SW
  TITLE     Structural Basis of the Heterodimer Formation between Cell 
            Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori.
  JOURNAL   PLoS One. 2016 Oct 6;11(10):e0164243. doi: 
            10.1371/journal.pone.0164243. eCollection 2016.
  PUBMED    27711177
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-10-6"; volume: "11(10):e0164243. doi"; pages: " 
            10.1371/journal.pone.0164243. eCollection 2016"
COMMENT     Sequence Label: csd1_125-312/pET28b(+)
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(629..646)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(656..673)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             692..714
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    729..753
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(753..772)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(754..772)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(856..875)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtt
       61 tttgtgcctt gagcgatgat agtcgctggt cctgtttttg cattagccga tttattattg
      121 ttatcaaaga ttgccatctg atgctccttt ctttattgaa aacttcttca aaatctttca
      181 tgttccattt ggtgaaactc atggggttta tgggttgatc taaaaaacgc acttcataat
      241 gcaaatgcgg ccctgtgctc atccctgtat taccactata cccaatcaac tgcccttttt
      301 tgacaaattc gcccgttttt acgacgattt tattcaaatg ggcgtagtag gttttaaaac
      361 caaaagggtg gaaaacttta atcaaattcc catacccccc attccacccc ttgctcgcta
      421 accctactac cccgctcgcg ctcgcataca caggggtgtt aatagcggtg cttaaatcaa
      481 gcccggtatg gttgtgcagc acatgcaaaa tagggtggat tcttttatta aaggcggctg
      541 aaacgcgccg ataggattct agcgggtagt cattagggat aaggcgcatg ataaagcttt
      601 tttgaagccc ggtaatccca gccatatggc tgccgcgcgg caccaggccg ctgctgtgat
      661 gatgatgatg atggctgctg cccatggtat atctccttct taaagttaaa caaaattatt
      721 tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg
      781 ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
      841 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
      901 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
      961 tgttgggcgc catc
//
