LOCUS       sequence-164286-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd2, construct: 121-308, pET28b(+) NC-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, 
            Mobashery S, Kim SJ, Suh SW
  TITLE     Structural Basis of the Heterodimer Formation between Cell 
            Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori.
  JOURNAL   PLoS One. 2016 Oct 6;11(10):e0164243. doi: 
            10.1371/journal.pone.0164243. eCollection 2016.
  PUBMED    27711177
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-10-6"; volume: "11(10):e0164243. doi"; pages: " 
            10.1371/journal.pone.0164243. eCollection 2016"
COMMENT     Sequence Label: csd2_121-308/pET28b(+)
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(628..645)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(655..672)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             691..713
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    728..752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(752..771)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(753..771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(855..874)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttcctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagctg
       61 gcttatgagt gcgtctttat ccacatgctc ttggagctgg acaatgtctt ctaaaaccca
      121 aaacaaattc ttccattcaa taaatttatt ttcttctaaa gcgctttgaa aatgatccaa
      181 atcccatgcc agaaattttt gcgcgtctaa aattttaccc aaaaagcgca cttcataatg
      241 caatttttcg ccgccgctat taccgctctt cccgctatag ccaatcaact gccctttttg
      301 gatgaagctt ttgggctgca cattgacatg atctaagtgc gtgtaaatgg agctaaaacc
      361 aaacgcatgt tcaatgcgca ccaagttccc ataccccaca ttagaattgg tcttcacaaa
      421 atccacaatc ccatcagcgc tcgcataaac aggcgtgttt aatggcgcga taaaatcaat
      481 cccggattca acgcccttaa tttttttaat ggggtggttc ctttctttgg tgggtttgat
      541 agcgctataa gtttttatgg gcatgccgtt aggaatgagc atgagcgcta aatgtttttg
      601 agccagattc aaattgtcta acatatggct gccgcgcggc accaggccgc tgctgtgatg
      661 atgatgatga tggctgctgc ccatggtata tctccttctt aaagttaaac aaaattattt
      721 ctagagggga attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg
      781 gatcgagatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
      841 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
      901 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
      961 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg g
//
