LOCUS       sequence-164291-        4224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ETV1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ETV1_WT
FEATURES             Location/Qualifiers
     source          1..4224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1624..1723)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1740..1759)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1741..1759)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1764..1780)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1764..1780)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2211..3002
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3095..3683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3584..3603
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3837..3854
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4013..4040)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4132..4218)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggatgga ttttatgacc agcaagtgcc ttacatggtc accaatagtc
      241 agcgtgggag aaattgtaac gagaaaccaa caaatgtcag gaaaagaaaa ttcattaaca
      301 gagatctggc tcatgattca gaagaactct ttcaagatct aagtcaatta caggaaacat
      361 ggcttgcaga agctcaggta cctgacaatg atgagcagtt tgtaccagac tatcaggctg
      421 aaagtttggc ttttcatggc ctgccactga aaatcaagaa agaaccccac agtccatgtt
      481 cagaaatcag ctctgcctgc agtcaagaac agccctttaa attcagctat ggagaaaagt
      541 gcctgtacaa tgtcagtgcc tatgatcaga agccacaagt gggaatgagg ccctccaacc
      601 cccccacacc atccagcacg ccagtgtccc cactgcatca tgcatctcca aactcaactc
      661 atacaccgaa acctgaccgg gccttcccag ctcacctccc tccatcgcag tccataccag
      721 atagcagcta ccccatggac cacagatttc gccgccagct ttctgaaccc tgtaactcct
      781 ttcctccttt gccgacgatg ccaagggaag gacgtcctat gtaccaacgc cagatgtctg
      841 agccaaacat ccccttccca ccacaaggct ttaagcagga gtaccacgac ccagtgtatg
      901 aacacaacac catggttggc agtgcggcca gccaaagctt tccccctcct ctgatgatta
      961 aacaggaacc cagagatttt gcatatgact cagaagtgcc tagctgccac tccatttata
     1021 tgaggcaaga aggcttcctg gctcatccca gcagaacaga aggctgtatg tttgaaaagg
     1081 gccccaggca gttttatgat gacacctgtg ttgtcccaga aaaattcgat ggagacatca
     1141 aacaagagcc aggaatgtat cgggaaggac ccacatacca acggcgagga tcacttcagc
     1201 tctggcagtt tttggtagct cttctggatg acccttcaaa ttctcatttt attgcctgga
     1261 ctggtcgagg catggaattt aaactgattg agcctgaaga ggtggcccga cgttggggca
     1321 ttcagaaaaa caggccagct atgaactatg ataaacttag ccgttcactc cgctattact
     1381 atgagaaagg aattatgcaa aaggtggctg gagagagata tgtctacaag tttgtgtgtg
     1441 atccagaagc ccttttctcc atggcctttc cagataatca gcgtccactg ctgaagacag
     1501 acatggaacg tcacatcaac gaggaggaca cagtgcctct ttctcacttt gatgagagca
     1561 tggcctacat gccggaaggg ggctgctgca acccccaccc ctacaacgaa ggctacgtgt
     1621 atttgccaac tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     1681 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     1741 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     1801 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
     1861 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     1921 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     1981 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     2041 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     2101 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     2161 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     2221 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     2281 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     2341 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     2401 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     2461 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     2521 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     2581 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     2641 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     2701 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     2761 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     2821 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     2881 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     2941 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     3001 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     3061 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3121 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3181 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3241 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3301 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3361 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3421 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3481 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3541 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3601 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3661 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3721 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3781 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3841 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     3901 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     3961 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     4021 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     4081 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     4141 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     4201 agcctttcgt tttatttgat gcct
//
