LOCUS       sequence-164292-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd2, construct: 121-308, pET28b(+) NC-tag, mutation: E165A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, 
            Mobashery S, Kim SJ, Suh SW
  TITLE     Structural Basis of the Heterodimer Formation between Cell 
            Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori.
  JOURNAL   PLoS One. 2016 Oct 6;11(10):e0164243. doi: 
            10.1371/journal.pone.0164243. eCollection 2016.
  PUBMED    27711177
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-10-6"; volume: "11(10):e0164243. doi"; pages: " 
            10.1371/journal.pone.0164243. eCollection 2016"
COMMENT     Sequence Label: csd2_121-308,E165A/pET28b(+)
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             667..687
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     primer_bind     complement(740..758)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      754..801
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(925..944)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 taattttgtt taactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggtgcc gcgcggcagc catatgttag acaatttgaa tctggctcaa
      121 aaacatttag cgctcatgct cattcctaac ggcatgccca taaaaactta tagcgctatc
      181 aaacccacca aagaaaggaa ccaccccatt aaaaaaatta agggcgttgc atccgggatt
      241 gattttatcg cgccattaaa cacgcctgtt tatgcgagcg ctgatgggat tgtggatttt
      301 gtgaagacca attctaatgt ggggtatggg aacttggtgc gcattgaaca tgcgtttggt
      361 tttagctcca tttacacgca cttagatcat gtcaatgtgc agcccaaaag cttcatccaa
      421 aaagggcagt tgattggcta tagcgggaag agcggtaata gcggcggcga aaaattgcat
      481 tatgaagtgc gctttttggg taaaatttta gacgcgcaaa aatttctggc atgggatttg
      541 gatcattttc aaagcgcttt agaagaaaat aaatttattg aatggaagaa tttgttttgg
      601 gttttagaag acattgtcca gctccaagag catgtggata aagacgcact cataagccag
      661 ctcgagcacc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa
      721 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      781 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg
      841 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      901 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
      961 tcgccggctt tccccgtcaa gct
//
