LOCUS       sequence-164293-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd2, construct: 121-308, pET28b(+) NC-tag, mutation: E165A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, 
            Mobashery S, Kim SJ, Suh SW
  TITLE     Structural Basis of the Heterodimer Formation between Cell 
            Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori.
  JOURNAL   PLoS One. 2016 Oct 6;11(10):e0164243. doi: 
            10.1371/journal.pone.0164243. eCollection 2016.
  PUBMED    27711177
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-10-6"; volume: "11(10):e0164243. doi"; pages: " 
            10.1371/journal.pone.0164243. eCollection 2016"
COMMENT     Sequence Label: csd2_121-308,E165A/pET28b(+)
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             complement(627..644)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(654..671)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             690..712
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    727..751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(751..770)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(752..770)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(854..873)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtggtg ctcgagctgg
       61 cttatgagtg cgtctttatc cacatgctct tggagctgga caatgtcttc taaaacccaa
      121 aacaaattct tccattcaat aaatttattt tcttctaaag cgctttgaaa atgatccaaa
      181 tcccatgcca gaaatttttg cgcgtctaaa attttaccca aaaagcgcac ttcataatgc
      241 aatttttcgc cgccgctatt accgctcttc ccgctatagc caatcaactg ccctttttgg
      301 atgaagcttt tgggctgcac attgacatga tctaagtgcg tgtaaatgga gctaaaacca
      361 aacgcatgtt caatgcgcac caagttccca taccccacat tagaattggt cttcacaaaa
      421 tccacaatcc catcagcgct cgcataaaca ggcgtgttta atggcgcgat aaaatcaatc
      481 ccggatgcaa cgcccttaat ttttttaatg gggtggttcc tttctttggt gggtttgata
      541 gcgctataag tttttatggg catgccgtta ggaatgagca tgagcgctaa atgtttttga
      601 gccagattca aattgtctaa catatggctg ccgcgcggca ccaggccgct gctgtgatga
      661 tgatgatgat ggctgctgcc catggtatat ctccttctta aagttaaaca aaattatttc
      721 tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg
      781 atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      841 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      901 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgt
//
