LOCUS       sequence-164295-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd2, construct: 121-308, pET28b(+) NC-tag, mutation: Y197F.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, 
            Mobashery S, Kim SJ, Suh SW
  TITLE     Structural Basis of the Heterodimer Formation between Cell 
            Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori.
  JOURNAL   PLoS One. 2016 Oct 6;11(10):e0164243. doi: 
            10.1371/journal.pone.0164243. eCollection 2016.
  PUBMED    27711177
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-10-6"; volume: "11(10):e0164243. doi"; pages: " 
            10.1371/journal.pone.0164243. eCollection 2016"
COMMENT     Sequence Label: csd2_121-308,Y197F/pET28b(+)
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..97
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             674..691
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(744..762)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      758..805
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(929..948)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca
       61 tcatcatcac agcagcggcc tggtgccgcg cggcagccat atgttagaca atttgaatct
      121 ggctcaaaaa catttagcgc tcatgctcat tcctaacggc atgcccataa aaacttatag
      181 cgctatcaaa cccaccaaag aaaggaacca ccccattaaa aaaattaagg gcgttgaatc
      241 cgggattgat tttatcgcgc cattaaacac gcctgtttat gcgagcgctg atgggattgt
      301 ggattttgtg aagaccaatt ctaatgtggg gtttgggaac ttggtgcgca ttgaacatgc
      361 gtttggtttt agctccattt acacgcactt agatcatgtc aatgtgcagc ccaaaagctt
      421 catccaaaaa gggcagttga ttggctatag cgggaagagc ggtaatagcg gcggcgaaaa
      481 attgcattat gaagtgcgct ttttgggtaa aattttagac gcgcaaaaat ttctggcatg
      541 ggatttggat cattttcaaa gcgctttaga agaaaataaa tttattgaat ggaagaattt
      601 gttttgggtt ttagaagaca ttgtccagct ccaagagcat gtggataaag acgcactcat
      661 aagccagctc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa
      721 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      781 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg
      841 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      901 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      961 acgttcgccg gctttccccg tcaagctcta aatcggggg
//
