LOCUS       sequence-164296-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd2, construct: 121-308, pET28b(+) NC-tag, mutation: Y197F.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, 
            Mobashery S, Kim SJ, Suh SW
  TITLE     Structural Basis of the Heterodimer Formation between Cell 
            Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori.
  JOURNAL   PLoS One. 2016 Oct 6;11(10):e0164243. doi: 
            10.1371/journal.pone.0164243. eCollection 2016.
  PUBMED    27711177
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-10-6"; volume: "11(10):e0164243. doi"; pages: " 
            10.1371/journal.pone.0164243. eCollection 2016"
COMMENT     Sequence Label: csd2_121-308,Y197F/pET28b(+)
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(625..642)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(652..669)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             688..710
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    725..749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(749..768)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(750..768)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(852..871)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagctggct
       61 tatgagtgcg tctttatcca catgctcttg gagctggaca atgtcttcta aaacccaaaa
      121 caaattcttc cattcaataa atttattttc ttctaaagcg ctttgaaaat gatccaaatc
      181 ccatgccaga aatttttgcg cgtctaaaat tttacccaaa aagcgcactt cataatgcaa
      241 tttttcgccg ccgctattac cgctcttccc gctatagcca atcaactgcc ctttttggat
      301 gaagcttttg ggctgcacat tgacatgatc taagtgcgtg taaatggagc taaaaccaaa
      361 cgcatgttca atgcgcacca agttcccaaa ccccacatta gaattggtct tcacaaaatc
      421 cacaatccca tcagcgctcg cataaacagg cgtgtttaat ggcgcgataa aatcaatccc
      481 ggattcaacg cccttaattt ttttaatggg gtggttcctt tctttggtgg gtttgatagc
      541 gctataagtt tttatgggca tgccgttagg aatgagcatg agcgctaaat gtttttgagc
      601 cagattcaaa ttgtctaaca tatggctgcc gcgcggcacc aggccgctgc tgtgatgatg
      661 atgatgatgg ctgctgccca tggtatatct ccttcttaaa gttaaacaaa attatttcta
      721 gaggggaatt gttatccgct cacaattccc ctatagtgag tcgtattaat ttcgcgggat
      781 cgagatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
      841 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
      901 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
      961 gggcgccatc tccttgcatg caccattcct tgc
//
