LOCUS       sequence-164297-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  doxycycline-inducible lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Panciera T, Azzolin L, Fujimura A, Di Biagio D, Frasson C, Bresolin 
            S, Soligo S, Basso G, Bicciato S, Rosato A, Cordenonsi M, Piccolo S
  TITLE     Induction of Expandable Tissue-Specific Stem/Progenitor Cells 
            through Transient Expression of YAP/TAZ.
  JOURNAL   Cell Stem Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 
            10.1016/j.stem.2016.08.009.
  PUBMED    27641305
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 
            10.1016/j.stem.2016.08.009."
COMMENT     Sequence Label: FUW-tetO-MCS
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    53..174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(64..83)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     118..137
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(304..678)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(697..744)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      806..941
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(860..879)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 tgaccatgat tacgccaagc tctagctaga ggtcgacggt atacagacat gataagatac
       61 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
      121 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttggggtg
      181 ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa
      241 aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtagcac
      301 gtgtcagtcc tgctcctcgg ccacgaagtg cacgcagttg ccggccgggt cgcgcagggc
      361 gaactcccgc ccccacggct gctcgccgat ctcggtcatg gccggcccgg aggcgtcccg
      421 gaagttcgtg gacacgacct ccgaccactc ggcgtacagc tcgtccaggc cgcgcaccca
      481 cacccaggcc agggtgttgt ccggcaccac ctggtcctgg accgcgctga tgaacagggt
      541 cacgtcgtcc cggaccacac cggcgaagtc gtcctccacg aagtcccggg agaacccgag
      601 ccggtcggtc cagaactcga ccgctccggc gacgtcgcgc gcggtgagca ccggaacggc
      661 actggtcaac ttggccatgg tttagttcct caccttgtcg tattatacta tgccgatata
      721 ctatgccgat gattaattgt caacacgtgc tgatcagatc cgaaaatgga tatacaagct
      781 cccgggagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat
      841 agctcagagg cagaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
      901 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcgg
//
