LOCUS       sequence-164298-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  doxycycline-inducible lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Panciera T, Azzolin L, Fujimura A, Di Biagio D, Frasson C, Bresolin 
            S, Soligo S, Basso G, Bicciato S, Rosato A, Cordenonsi M, Piccolo S
  TITLE     Induction of Expandable Tissue-Specific Stem/Progenitor Cells 
            through Transient Expression of YAP/TAZ.
  JOURNAL   Cell Stem Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 
            10.1016/j.stem.2016.08.009.
  PUBMED    27641305
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 
            10.1016/j.stem.2016.08.009."
COMMENT     Sequence Label: FUW-tetO-MCS
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(235..259)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(256..294)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(327..345,346..368,369..387,388..410,411..429,430..452,
                     453..471,472..494,495..513,514..536,537..555,556..578,
                     579..597)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     misc_feature    650..767
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat
       61 tgacatgagt cacatctaga actggttaac tcatggccct tgaggcccaa gagaattcta
      121 tctggcgcgc ctcgtaggat cctgatagct agcctgtaat tcggggccgc ggaggctgga
      181 tcggtcccgg tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg
      241 acggttcact aaacgagctc tgcttatata ggcctcccac cgtacacgcc tacctcgacc
      301 cgggtaccga gctcgacttt cacttttctc tatcactgat agggagtggt aaactcgact
      361 ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac
      421 tgatagggag tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac
      481 tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc
      541 tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg
      601 gtaaactcga taaccaaact ggatctctgc tgtccctgta ataaacccga aaattttgaa
      661 tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta
      721 ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata
      781 ctgccatttg tctgcagaat tggcgcacgc agtgccgatc cgttcactaa tcgaatggat
      841 ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc gggtcccctc
      901 ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact
      961 atagaaagta cagcaaaaac tattctta
//
