LOCUS       sequence-164303-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd2, construct: 121-308, pET28b(+) NC-tag, mutation: T151A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, 
            Mobashery S, Kim SJ, Suh SW
  TITLE     Structural Basis of the Heterodimer Formation between Cell 
            Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori.
  JOURNAL   PLoS One. 2016 Oct 6;11(10):e0164243. doi: 
            10.1371/journal.pone.0164243. eCollection 2016.
  PUBMED    27711177
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-10-6"; volume: "11(10):e0164243. doi"; pages: " 
            10.1371/journal.pone.0164243. eCollection 2016"
COMMENT     Sequence Label: csd2_121-308,T151A/pET28b(+)
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..98
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             675..692
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(745..763)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      759..806
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(930..949)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac catgggcagc agccatcatc
       61 atcatcatca cagcagcggc ctggtgccgc gcggcagcca tatgttagac aatttgaatc
      121 tggctcaaaa acatttagcg ctcatgctca ttcctaacgg catgcccata aaaacttata
      181 gcgctatcaa acccgccaaa gaaaggaacc accccattaa aaaaattaag ggcgttgaat
      241 ccgggattga ttttatcgcg ccattaaaca cgcctgttta tgcgagcgct gatgggattg
      301 tggattttgt gaagaccaat tctaatgtgg ggtatgggaa cttggtgcgc attgaacatg
      361 cgtttggttt tagctccatt tacacgcact tagatcatgt caatgtgcag cccaaaagct
      421 tcatccaaaa agggcagttg attggctata gcgggaagag cggtaatagc ggcggcgaaa
      481 aattgcatta tgaagtgcgc tttttgggta aaattttaga cgcgcaaaaa tttctggcat
      541 gggatttgga tcattttcaa agcgctttag aagaaaataa atttattgaa tggaagaatt
      601 tgttttgggt tttagaagac attgtccagc tccaagagca tgtggataaa gacgcactca
      661 taagccagct cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
      721 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      781 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg
      841 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      901 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      961 cacgttcgcc ggctttcccc gtcaagct
//
