LOCUS       sequence-164309-        9983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses wtYAP in mammalian cells under the control of a 
            doxycycline-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Panciera T, Azzolin L, Fujimura A, Di Biagio D, Frasson C, Bresolin 
            S, Soligo S, Basso G, Bicciato S, Rosato A, Cordenonsi M, Piccolo S
  TITLE     Induction of Expandable Tissue-Specific Stem/Progenitor Cells 
            through Transient Expression of YAP/TAZ.
  JOURNAL   Cell Stem Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 
            10.1016/j.stem.2016.08.009.
  PUBMED    27641305
REFERENCE   2  (bases 1 to 9983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 
            10.1016/j.stem.2016.08.009."
COMMENT     Sequence Label: FUW-tetO-wtYAP
FEATURES             Location/Qualifiers
     source          1..9983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2399..2417,2418..2440,2441..2459,2460..2482,2483..2501,
                     2502..2524,2525..2543,2544..2566,2567..2585,2586..2608,
                     2609..2627,2628..2650,2651..2669)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2702..2740
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2737..2761
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2884..2893
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2893..2916
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4464..5052
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4517..4537)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4935..4946)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5055..5071)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5056..5072)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5577..5757
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5783..5800)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5789..6013
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6059..6487
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6146..6165)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6356..6377
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6496..6516)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6501..6830
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6681..6816
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6743..6762
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6878..6925
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6944..7318
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7448..7569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7485..7504)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7539..7558
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7618..7634)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7618..7634)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7631..7653)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7642..7658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7666..7672,7673..7690,7691..7696))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7711..7732
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7849..7866)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8020..8608)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8100..8119)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8779..9570,9571..9639))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9402..9421
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9640..9744)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9819..9838)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttatcgag tttaccactc
     2401 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     2461 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     2521 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     2581 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     2641 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagctcggt acccgggtcg
     2701 aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
     2761 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     2821 cctccgcggc cccgaattac aggctagcta tcaggatcct acgaggcgcg ccagatagaa
     2881 ttcgccacca tggactacaa ggacgacgac gacaaggatc ccgggcagca gccgccgcct
     2941 caaccggccc cccagggcca agggcagccg ccttcgcagc ccccgcaggg gcagggcccg
     3001 ccgtccggac ccgggcaacc ggcacccgcg gcgacccagg cggcgccgca ggcacccccc
     3061 gccgggcatc agatcgtgca cgtccgcggg gactcggaga ccgacctgga ggcgctcttc
     3121 aacgccgtca tgaaccccaa gacggccaac gtgccccaga ccgtgcccat gaggctccgg
     3181 aagctgcccg actccttctt caagccgccg gagcccaaat cccactcccg acaggccagt
     3241 actgatgcag gcactgcagg agccctgact ccacagcatg ttcgagctca ttcctctcca
     3301 gcttctctgc agttgggagc tgtttctcct gggacactga cccccactgg agtagtctct
     3361 ggcccagcag ctacacccac agctcagcat cttcgacagt cttcttttga gatacctgat
     3421 gatgtacctc tgccagcagg ttgggagatg gcaaaaacca gcagcgggca acgttatttt
     3481 ctcaatcaca tcgatcagac aacaacatgg caggacccca ggaaggccat gctgtcccag
     3541 atgaacgtca cagcccccac cagtccacca gtgcagcaga atatgatgaa ctcggcttca
     3601 ggtcctcttc ctgatggatg ggaacaagcc atgactcagg atggagaaat ttactatata
     3661 aaccataaga acaagaccac ctcttggcta gacccaaggc ttgaccctcg ttttgccatg
     3721 aaccagagaa tcagtcagag tgctccagtg aaacagccac cacccctggc tccccagagc
     3781 ccacagggag gcgtcatggg tggcagcaac tccaaccagc agcaacagat gcgactgcag
     3841 caactgcaga tggagaagga gaggctgcgg ctgaaacagc aagaactgct tcggcaggca
     3901 atgcggaata tcaatcccag cacagcaaat tctccaaaat gtcaggagtt agccctgcgt
     3961 agccagttac caacactgga gcaggatggt gggactcaaa atccagtgtc ttctcccggg
     4021 atgtctcagg aattgagaac aatgacgacc aatagctcag atcctttcct taacagtggc
     4081 acctatcact ctcgagatga gagtacagac agtggactaa gcatgagcag ctacagtgtc
     4141 cctcgaaccc cagatgactt cctgaacagt gtggatgaga tggatacagg tgatactatc
     4201 aaccaaagca ccctgccctc acagcagaac cgtttcccag actaccttga agccattcct
     4261 gggacaaatg tggaccttgg aacactggaa ggagatggaa tgaacataga aggagaggag
     4321 ctgatgccaa gtctgcagga agctttgagt tctgacatcc ttaatgacat ggagtctgtt
     4381 ttggctgcca ccaagctaga taaagaaagc tttcttacat ggttatagag cggccatgtc
     4441 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     4501 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4561 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4621 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4681 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4741 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4801 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     4861 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     4921 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4981 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     5041 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa
     5101 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     5161 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     5221 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     5281 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     5341 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     5401 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     5461 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     5521 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     5581 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5641 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5701 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     5761 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     5821 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     5881 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     5941 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6001 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     6061 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6121 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6181 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6241 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6301 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6361 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6421 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6481 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6541 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6601 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6661 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6721 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     6781 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     6841 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     6901 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     6961 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     7021 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     7081 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     7141 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     7201 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     7261 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     7321 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7381 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     7441 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7501 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7561 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     7621 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7681 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7741 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7801 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7861 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7921 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7981 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8041 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8101 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8161 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8221 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8281 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8341 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8401 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8461 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8521 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8581 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8641 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8701 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8761 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8821 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8881 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8941 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9001 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9061 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9121 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9181 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9241 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9301 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9361 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9421 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9481 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9541 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9601 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9661 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9721 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     9781 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     9841 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     9901 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     9961 gttaggcgtt ttgcgctgct tcg
//
