LOCUS       sequence-164311-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses a TEAD-binding mutant of YAP (YAPS94A) in mammalian cells 
            under the control of a doxycycline-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Panciera T, Azzolin L, Fujimura A, Di Biagio D, Frasson C, Bresolin 
            S, Soligo S, Basso G, Bicciato S, Rosato A, Cordenonsi M, Piccolo S
  TITLE     Induction of Expandable Tissue-Specific Stem/Progenitor Cells 
            through Transient Expression of YAP/TAZ.
  JOURNAL   Cell Stem Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 
            10.1016/j.stem.2016.08.009.
  PUBMED    27641305
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 
            10.1016/j.stem.2016.08.009."
COMMENT     Sequence Label: FUW-tetO-YAPS94A
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      109..118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             118..141
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga
       61 attacaggct agctatcagg atcctacgag gcgcgccaga tagaattcgc caccatggac
      121 tacaaggacg acgacgacaa ggatcccggg cagcagccgc cgcctcaacc ggccccccag
      181 ggccaagggc agccgccttc gcagcccccg caggggcagg gcccgccgtc cggacccggg
      241 caaccggcac ccgcggcgac ccaggcggcg ccgcaggcac cccccgccgg gcatcagatc
      301 gtgcacgtcc gcggggactc ggagaccgac ctggaggcgc tcttcaacgc cgtcatgaac
      361 cccaagacgg ccaacgtgcc ccagaccgtg cccatgaggc tccggaagct gcccgacgcg
      421 ttcttcaagc cgccggagcc caaatcccac tcccgacagg ccagtactga tgcaggcact
      481 gcaggagccc tgactccaca gcatgttcga gctcattcct ctccagcttc tctgcagttg
      541 ggagctgttt ctcctgggac actgaccccc actggagtag tctctggccc agcagctaca
      601 cccacagctc agcatcttcg acagtcttct tttgagatac ctgatgatgt acctctgcca
      661 gcaggttggg agatggcaaa aaccagcagc gggcaacgtt attttctcaa tcacatcgat
      721 cagacaacaa catggcagga ccccaggaag gccatgctgt cccagatgaa cgtcacagcc
      781 cccaccagtc caccagtgca gcagaatatg atgaactcgg cttcaggtcc tcttcctgat
      841 ggatgggaac aagccatgac tcaggatgga gaaatttact atataaacca taagaacaag
      901 accacctctt ggctagaccc aaggcttgac cctcgttttg ccatgaacca gagaatcagt
      961 cagagtgctc cagtgaaaca gc
//
