LOCUS       sequence-164315-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd2, construct: 121-308, pET28b(+) NC-tag, mutation: S166A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, 
            Mobashery S, Kim SJ, Suh SW
  TITLE     Structural Basis of the Heterodimer Formation between Cell 
            Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori.
  JOURNAL   PLoS One. 2016 Oct 6;11(10):e0164243. doi: 
            10.1371/journal.pone.0164243. eCollection 2016.
  PUBMED    27711177
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-10-6"; volume: "11(10):e0164243. doi"; pages: " 
            10.1371/journal.pone.0164243. eCollection 2016"
COMMENT     Sequence Label: csd2_121-308,S166A/pET28b(+)
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             59..76
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..103
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             680..697
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(750..768)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      764..811
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(935..954)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca
       61 tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatgt tagacaattt
      121 gaatctggct caaaaacatt tagcgctcat gctcattcct aacggcatgc ccataaaaac
      181 ttatagcgct atcaaaccca ccaaagaaag gaaccacccc attaaaaaaa ttaagggcgt
      241 tgaagccggg attgatttta tcgcgccatt aaacacgcct gtttatgcga gcgctgatgg
      301 gattgtggat tttgtgaaga ccaattctaa tgtggggtat gggaacttgg tgcgcattga
      361 acatgcgttt ggttttagct ccatttacac gcacttagat catgtcaatg tgcagcccaa
      421 aagcttcatc caaaaagggc agttgattgg ctatagcggg aagagcggta atagcggcgg
      481 cgaaaaattg cattatgaag tgcgcttttt gggtaaaatt ttagacgcgc aaaaatttct
      541 ggcatgggat ttggatcatt ttcaaagcgc tttagaagaa aataaattta ttgaatggaa
      601 gaatttgttt tgggttttag aagacattgt ccagctccaa gagcatgtgg ataaagacgc
      661 actcataagc cagctcgagc accaccacca ccaccactga gatccggctg ctaacaaagc
      721 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
      781 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc
      841 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      901 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      961 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggc
//
