LOCUS       sequence-164316-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd2, construct: 121-308, pET28b(+) NC-tag, mutation: S166A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, 
            Mobashery S, Kim SJ, Suh SW
  TITLE     Structural Basis of the Heterodimer Formation between Cell 
            Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori.
  JOURNAL   PLoS One. 2016 Oct 6;11(10):e0164243. doi: 
            10.1371/journal.pone.0164243. eCollection 2016.
  PUBMED    27711177
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-10-6"; volume: "11(10):e0164243. doi"; pages: " 
            10.1371/journal.pone.0164243. eCollection 2016"
COMMENT     Sequence Label: csd2_121-308,S166A/pET28b(+)
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(629..646)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(656..673)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             692..714
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    729..753
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(753..772)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(754..772)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(856..875)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagct
       61 ggcttatgag tgcgtcttta tccacatgct cttggagctg gacaatgtct tctaaaaccc
      121 aaaacaaatt cttccattca ataaatttat tttcttctaa agcgctttga aaatgatcca
      181 aatcccatgc cagaaatttt tgcgcgtcta aaattttacc caaaaagcgc acttcataat
      241 gcaatttttc gccgccgcta ttaccgctct tcccgctata gccaatcaac tgcccttttt
      301 ggatgaagct tttgggctgc acattgacat gatctaagtg cgtgtaaatg gagctaaaac
      361 caaacgcatg ttcaatgcgc accaagttcc cataccccac attagaattg gtcttcacaa
      421 aatccacaat cccatcagcg ctcgcataaa caggcgtgtt taatggcgcg ataaaatcaa
      481 tcccggcttc aacgccctta atttttttaa tggggtggtt cctttctttg gtgggtttga
      541 tagcgctata agtttttatg ggcatgccgt taggaatgag catgagcgct aaatgttttt
      601 gagccagatt caaattgtct aacatatggc tgccgcgcgg caccaggccg ctgctgtgat
      661 gatgatgatg atggctgctg cccatggtat atctccttct taaagttaaa caaaattatt
      721 tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg
      781 ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
      841 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
      901 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
      961 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgc
//
