LOCUS       sequence-164339-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac Cassette contains puromycin and TK genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Ye L, Wang J, Beyer AI, Teque F, Cradick TJ, Qi Z, Chang JC, Bao G, 
            Muench MO, Yu J, Levy JA, Kan YW
  TITLE     Seamless modification of wild-type induced pluripotent stem cells to
            the natural CCR5Delta32 mutation confers resistance to HIV 
            infection.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9591-6. doi: 
            10.1073/pnas.1407473111. Epub 2014 Jun 9.
  PUBMED    24927590
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1407473111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-07-1- 1"; volume: "111"; issue: "26"; pages: "9591-6"
COMMENT     Sequence Label: pCAG-PuroDTK.Neo
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   30..92
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(397..416)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1007..1026
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 agcggccgca tacgcgtata ctagattaac cctagaaaga taatcatatt gtgacgtacg
       61 ttaaagataa tcatgcgtaa aattgacgca tgtgttttat cggtctgtat atcgaggttt
      121 atttattaat ttgaatagat attaagtttt attatattta cacttacata ctaataataa
      181 attcaacaaa caatttattt atgtttattt atttattaaa aaaaaacaaa aactcaaaat
      241 ttcttctata aagtaacaaa acttttaact agtgattggt ctagatcaga agaactcgtc
      301 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
      361 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
      421 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
      481 attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcatcgcc
      541 gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
      601 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
      661 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
      721 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
      781 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
      841 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg
      901 cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
      961 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     1021 gaatagc
//
