LOCUS       sequence-164340-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac Cassette contains puromycin and TK genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Ye L, Wang J, Beyer AI, Teque F, Cradick TJ, Qi Z, Chang JC, Bao G, 
            Muench MO, Yu J, Levy JA, Kan YW
  TITLE     Seamless modification of wild-type induced pluripotent stem cells to
            the natural CCR5Delta32 mutation confers resistance to HIV 
            infection.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9591-6. doi: 
            10.1073/pnas.1407473111. Epub 2014 Jun 9.
  PUBMED    24927590
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1407473111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-07-1- 1"; volume: "111"; issue: "26"; pages: "9591-6"
COMMENT     Sequence Label: pCAG-PuroDTK.Neo
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   10..44
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        418..797
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctagattaac cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg
       61 ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc
      121 ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa
      181 cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc
      241 atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat
      301 tttcttgtta tagatatcaa ctagaatgct agcatgggcc catctcgacc tacttcgtat
      361 agcatacatt atacgaagtt atgtgcgggc ctcttcgcta ttacgccagc gcgcgttgac
      421 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      481 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      541 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      601 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      661 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      721 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      781 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
      841 ccccctcc
//
