LOCUS       sequence-164342-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac cassette contains Neomycin, puromycin and TK genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Ye L, Wang J, Beyer AI, Teque F, Cradick TJ, Qi Z, Chang JC, Bao G, 
            Muench MO, Yu J, Levy JA, Kan YW
  TITLE     Seamless modification of wild-type induced pluripotent stem cells to
            the natural CCR5Delta32 mutation confers resistance to HIV 
            infection.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9591-6. doi: 
            10.1073/pnas.1407473111. Epub 2014 Jun 9.
  PUBMED    24927590
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1407473111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-07-1- 1"; volume: "111"; issue: "26"; pages: "9591-6"
COMMENT     Sequence Label: pCAG-Neo-PuroDTK
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   29..91
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    273..306
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    319..543
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     532..549
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gcggccgcat acgcgtatac tagattaacc ctagaaagat aatcatattg tgacgtacgt
       61 taaagataat catgcgtaaa attgacgcat gtgttttatc ggtctgtata tcgaggttta
      121 tttattaatt tgaatagata ttaagtttta ttatatttac acttacatac taataataaa
      181 ttcaacaaac aatttattta tgtttattta tttattaaaa aaaaacaaaa actcaaaatt
      241 tcttctataa agtaacaaaa cttttaacta gtgaagttcc tattctctag aaagtatagg
      301 aacttcccgc ctcagaagcc atagagccca ccgcatcccc agcatgcctg ctattgtctt
      361 cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac
      421 tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt
      481 ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca
      541 cagtcgaggc tgatcagcga gctctagagc tcagttagcc tcccccatct cccgggcaaa
      601 cgtgcgcgcc aggtcgcaga tcgtcggtat ggagccgggg gtggtgacgt gggtctggac
      661 catcccggag gtaagttgca gcagggcgtc ccggcagccg gcgggcgatt ggtcgtaatc
      721 caggataaag acgtgcatgg gacggaggcg tttggccaag acgtccaagg cccaggcaaa
      781 cacgttgtac aggtcgccgt tgggggccag caactcgggg gcccgaaaca gggtaaataa
      841 cgtgtccccg atatggggtc gtgggcccgc gttgctctgg ggctcggcac cctggggcgg
      901 cacgg
//
